The roles of ERAS during cell lineage specification of mouse early embryonic development.
The roles of ERAS during cell lineage specification of mouse early embryonic development.
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ERAS在小鼠早期胚胎发育细胞谱系规范中的作用
DOI：
10.1098/rsob.150092
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发表时间：
2015-08
期刊：
影响因子：
5.8
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Zhao ZA;Yu Y;Ma HX;Wang XX;Lu X;Zhai Y;Zhang X;Wang H;Li L
Eras编码一种Ras样GTPase蛋白，该蛋白最初被鉴定为胚胎干细胞特异性Ras。已知ERAS是胚胎干细胞生长所必需的，并刺激体细胞重编程，提示其在小鼠早期胚胎发育中的作用。我们现在报道了Eras在小鼠着床期发育过程中的动态表达模式：其表达在囊胚期增加，并在E7.5中胚层特异性降低。根据其表达模式，Eras的表达增加通过控制AKT的激活和通过ERK激活小鼠胚胎干细胞从ground到primed状态的承诺来促进细胞增殖；Eras表达的减少通过抑制原肠胚形成过程中的AKT促进了原始条纹和中胚层的形成。在小鼠早期胚胎发育过程中，Eras的表达受到β-catenin通路的负调控。因此，除了众所周知的细胞增殖作用外，ERAS还可能在小鼠早期胚胎发育期间的细胞谱系规范中发挥重要作用。
Eras encodes a Ras-like GTPase protein that was originally identified as an embryonic stem cell-specific Ras. ERAS has been known to be required for the growth of embryonic stem cells and stimulates somatic cell reprogramming, suggesting its roles on mouse early embryonic development. We now report a dynamic expression pattern of Eras during mouse peri-implantation development: its expression increases at the blastocyst stage, and specifically decreases in E7.5 mesoderm. In accordance with its expression pattern, the increased expression of Eras promotes cell proliferation through controlling AKT activation and the commitment from ground to primed state through ERK activation in mouse embryonic stem cells; and the reduced expression of Eras facilitates primitive streak and mesoderm formation through AKT inhibition during gastrulation. The expression of Eras is finely regulated to match its roles in mouse early embryonic development during which Eras expression is negatively regulated by the β-catenin pathway. Thus, beyond its well-known role on cell proliferation, ERAS may also play important roles in cell lineage specification during mouse early embryonic development.