Scavenger Receptor Collectin Placenta 1 (CL-P1) Predominantly Mediates Zymosan Phagocytosis by Human Vascular Endothelial Cells
Scavenger Receptor Collectin Placenta 1 (CL-P1) Predominantly Mediates Zymosan Phagocytosis by Human Vascular Endothelial Cells
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DOI：
10.1074/jbc.m807477200
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发表时间：
2009-02-06
影响因子：
4.8
通讯作者：
Wakamiya, Nobutaka
中科院分区：
文献类型：
--
作者：
Jang, SeongJae;Ohtani, Katsuki;Wakamiya, Nobutaka
胎盘凝集素1(CL-P1)是新近发现的一种清道夫受体，介导氧化低密度脂蛋白和微生物的摄取。在这项研究中，我们利用稳定表达人CL-P1的中国仓鼠卵巢细胞(CHO/CL-P1)和组成表达CL-P1的人血管内皮细胞，研究了CL-P1介导的酵母多糖生物颗粒的结合和摄取。CHO/CL-P1对酵母多糖的摄取依赖于细胞膜上表达的CL-P1的水平，并被细胞松弛素D和Wortmannin抑制。酵母多糖的结合也被其他清道夫受体的配体如聚(I)和葡聚糖硫酸盐所抑制。实时定量逆转录-聚合酶链式反应分析表明，在不同个体分离的人脐静脉内皮细胞中，其他清道夫受体LOX-1、Stabilin-2或具有胶原性结构的巨噬细胞受体(MARCO)均不表达。CL-P1小干扰RNA可抑制3种原代培养的血管内皮细胞对酵母多糖的摄取，而其他清道夫受体的小干扰RNA对这些细胞的酵母多糖摄取无影响。此外，我们还证实CL-P1在人和小鼠血管内皮层中都有表达。我们的结果表明，CL-P1主要介导血管内皮细胞对真菌的吞噬作用。
Collectin placenta 1 (CL-P1), a recently discovered scavenger receptor, mediates the uptake of oxidized low density lipoprotein and microbes. In this study, we investigated CL-P1-mediated binding and ingestion of yeast-derived zymosan bioparticles using Chinese hamster ovary (CHO) cells stably expressing human CL-P1 (CHO/CL-P1) and human vascular endothelial cells constitutively expressed CL-P1. The uptake of zymosan by CHO/CL-P1 was dependent upon the level of CL-P1 expressed on the membrane and was inhibited by cytochalasin D and wortmannin. The binding of zymosan was also inhibited by ligands of other scavenger receptors such as poly(I) and dextran sulfate. Real time reverse transcription-PCR analyses showed that other scavenger receptors, namely LOX-1, Stabilin-2, or macrophage receptor with collagenous structure (MARCO), were not expressed in human umbilical vein endothelial cells isolated from different individuals. Nonopsonic zymosan ingestion was inhibited in three primary cultured vascular endothelial cells, including different human umbilical vein endothelial cells from nine individuals treated with CL-P1 small interfering RNAs, although small interfering RNAs of other scavenger receptors had no effect on zymosan uptake in these cells. Furthermore, we confirmed that CL-P1 is expressed in human and murine vascular endothelial layers. Our results demonstrated that CL-P1 predominantly mediated phagocytosis for fungi in vascular endothelia.