Radioimmunoassay of a thymine glycol.
Radioimmunoassay of a thymine glycol.
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胸腺嘧啶二醇的放射免疫测定。
作者：
G. J. West;I. W. West;J. Ward
建立了一种灵敏、特异的胸腺嘧啶二羟基-5,6-二氢胸腺嘧啶（胸腺嘧啶乙二醇）放射免疫测定方法。在${\rm O} {{\rm s}}{\rm O}_{4}\text{-treated}$ DNA中可以检测到仅4飞摩尔的胸腺嘧啶乙二醇。未修饰的DNA只在最高浓度下发生交叉反应。该分析依赖于胸腺嘧啶乙二醇的绝对量和胸腺嘧啶在聚合物中改变的部分。这是由于抗体对多个胸腺嘧啶乙二醇位点具有高特异性，使用${\rm O}_{\rm s}}{\rm O}_{4}\text{-修饰的}$ poly （dA-dT）和一系列低胸苷酸证实了这一点。提出了一种校正这种依赖性的方法。在γ辐照的DNA中，G值为0.018时，可以检测到胸腺嘧啶乙二醇；讨论了产量低的原因。该分析只需要温和的化学处理，许多样品可以快速和容易地处理。
A sensitive and specific radioimmunoassay for 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol) was developed. As little as 4 femtomoles of thymine glycol can be detected in ${\rm O}_{{\rm s}}{\rm O}_{4}\text{-treated}$ DNA. Unmodified DNA cross-reacts only at the highest concentrations. The assay is dependent on both the absolute amount of thymine glycol and the fraction of thymine altered in the polymer. This is caused by the high specificity of the antibody for multiple thymine glycol sites, as confirmed using ${\rm O}_{{\rm s}}{\rm O}_{4}\text{-modified}$ poly (dA-dT) and a series of oligothymidylic acids. A method of correcting for this dependence is presented. Using this RIA thymine glycol is detected in γ-irradiated DNA at a G value of 0.018; the reasons for this low yield are discussed. The assay requires only mild chemical treatment and many samples can be quickly and easily handled.