A MODEL SYSTEM FOR PEPTIDE-HORMONE ACTION IN DIFFERENTIATION - INTERLEUKIN-2 INDUCES A B-LYMPHOMA TO TRANSCRIBE THE J-CHAIN GENE
A MODEL SYSTEM FOR PEPTIDE-HORMONE ACTION IN DIFFERENTIATION - INTERLEUKIN-2 INDUCES A B-LYMPHOMA TO TRANSCRIBE THE J-CHAIN GENE
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DOI：
10.1016/0092-8674(86)90625-2
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发表时间：
1986-11-21
期刊：
影响因子：
64.5
通讯作者：
KOSHLAND, ME
中科院分区：
文献类型：
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作者：
BLACKMAN, MA;TIGGES, MA;KOSHLAND, ME
生理水平的纯化T细胞激素，白介素2(IL-2)，刺激克隆小鼠B细胞系(BCL1)分泌五聚体IgM抗体。多肽激素通过特定的IL-2受体作用于细胞表面，并诱导J链基因5‘’染色质的变化，该变化与其转录和五聚体IgM组装所需的J链蛋白的产生相关。IL-2对细胞增殖无影响，对MU也无影响。重链基因转录。这些结果确定了IL-2在B细胞分化中的特定功能。此外，IL-2/BCL1系统为研究激素-受体相互作用产生的信号传递到细胞核并调节基因表达的机制提供了一个模型。
Physiological levels of a purified T cell hormone, interleukin 2 (IL-2), were found to stimulate a cloned murine B cell line (BCL1) to secrete pentamer IgM antibody. The peptide hormone acts at the cell surface via specific IL-2 receptors and induces changes in the 5'' chromatin of the J chain gene that correlate with its transcription and with the production of the J chain protein required for pentamer IgM assembly. There was no effect of the IL-2 on cell proliferation nor on .mu. heavy chain gene transcription. These results define a specific function for IL-2 in B cell differentiation. In addition, the IL-2/BCL1 system provides a model for examining the mechanism by which signals generated by hormone-receptor interaction are transmitted to the nucleus and regulate gene expression.