The two duplicated insecticyanin genes, ins-a and ins-b are differentially expressed in the tobacco hornworm, Manduca sexta.
The two duplicated insecticyanin genes, ins-a and ins-b are differentially expressed in the tobacco hornworm, Manduca sexta.
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两个重复的虫青素基因 ins-a 和 ins-b 在烟草天蛾 Manduca sexta 中差异表达。
DOI：
10.1093/nar/22.15.2945
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发表时间：
1994
影响因子：
14.9
通讯作者：
Riddiford,LM
中科院分区：
文献类型：
--
作者：
Li,WC;Riddiford,LM
利用昆虫花青素基因ins-aandins-b的3′端非编码区序列设计了两个基因特异性探针，研究了这两个基因在天蛾发育过程中的表达。这两个基因最初在刚孵化的一龄幼虫中转录，然后在表皮中表达，在每个幼虫摄食阶段在脂肪体中表达程度较低。在4龄和5龄幼虫的表皮中，这两种mRNA在蜕皮前不久出现，并在一天内积累到最高水平。随着幼虫表皮在5龄（末龄）第3天化蛹，INSa mRNA迅速下降，而INS-b mRNA则出现第二个积累高峰。在脂肪体中，这两个基因在4龄幼虫中的表达模式与表皮相似，只是表达量较低，且以ins-bmRNA为主。在末龄，onlyins-bmRNA的表达量显著增加。这些发现不仅揭示了这两个重复的昆虫花青素基因在表达模式上存在差异，而且首次证明了脂肪体也表达昆虫花青素基因。
Two gene-specific probes were generated from the unique sequences in the 3′ non-coding regions of the two insecticyanin genes,ins-aandins-bto study the developmental expression of these genes inManduca sexta. Both genes were initially transcribed in the freshly hatched first instar larvae and then expressed in the epidermis and to a lesser degree in the fat body during every larval feeding stage. In the epidermis of the 4th and 5th instar larvae, both mRNAs appeared shortly before ecdysis and accumulated to maximal levels within a day. As the larval epidermis became pupally committed on day 3 of the 5th (final) instar, INSa mRNA quickly decreased, whereas INS-b mRNA showed a second peak of accumulation. In the fat body, both genes showed a similar expression pattern within the 4th instar to that of the epidermis except that levels were lower andins-bmRNA dominated. In the final instar, onlyins-bmRNA was present in significant amount. These findings not only reveal that the two duplicated insecticyanin genes have diverged in their expression pattern but also demonstrate, for the first time, that fat body also expresses insecticyanin genes.