Oligoclonal T cell expansions in pulmonary lymphoproliferative disorders: demonstration of the frequent occurrence of oligoclonal T cells in human immunodeficiency virus-related lymphoid interstitial pneumonia.
Oligoclonal T cell expansions in pulmonary lymphoproliferative disorders: demonstration of the frequent occurrence of oligoclonal T cells in human immunodeficiency virus-related lymphoid interstitial pneumonia.
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肺淋巴增殖性疾病中的寡克隆 T 细胞扩增：证明寡克隆 T 细胞在人类免疫缺陷病毒相关淋巴样间质性肺炎中频繁发生。
DOI：
10.1164/ajrccm.165.2.2101141
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Weiden,MichaelD
中科院分区：
文献类型：
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作者：
Kurosu,Katsushi;Yumoto,Norio;Rom,WilliamN;Takiguchi,Yuichi;Jaishree,Jagirdai;Nakata,Koh;Tatsumi,Koichiro;Mikata,Aatsuo;Kuriyama,Takatyuki;Weiden,MichaelD
我们采用变性梯度凝胶电泳（DGGE）技术，在聚合酶链反应（PCR）和测序分析中，使用富含40个核苷酸的鸟嘌呤和胞嘧啶序列，分析了肺淋巴组织增生性疾病中浸润性T淋巴细胞的T细胞抗原受体（TCR）-V γ基因库。 15例低度恶性粘膜相关淋巴组织（MALT）淋巴瘤和15例淋巴细胞性间质性肺炎（LIP）中分别有6例和8例出现TCR V γ基因的寡克隆区带。 DGGE测序结果显示，PCR扩增产物中存在多个TCR V γ寡克隆，提示传统的抗原特异性寡克隆扩增可能在肺淋巴组织增生性疾病的发病机制中起一定作用。人类免疫缺陷病毒（HIV）相关LIP中寡克隆浸润性T细胞扩增的频率（100%）显著高于低度肺MALT淋巴瘤（40%）或HIV阴性LIP（30%）。因为最近的证据表明，V3环的前病毒的氨基酸序列的单核细胞从支气管肺泡灌洗比外周血更均匀，这种均匀性可能会导致寡克隆扩增的浸润性T淋巴细胞作为一个后果，对肺特异性病毒株的持续反应。
We used a denaturing gradient gel electrophoresis (DGGE) procedure with 40-nucleotide guanine- and cytosine-rich sequences in the polymerase chain reaction (PCR) and sequencing analysis to analyze the T cell antigen receptor (TCR)-V γ gene repertoire of infiltrating T lymphocytes in pulmonary lymphoproliferative disorders. Six of 15 low-grade mucosa-associated lymphoid tissue (MALT) lymphomas and 8 of 15 cases of lymphocytic interstitial pneumonia (LIP) showed some oligoclonal bands for TCR-V γ genes on DGGE. Sequencing analysis demonstrated plural oligoclonal TCR-V γ clones among the oligoclonal PCR products on DGGE, leading to the conclusion that conventional antigen-specific oligoclonal expansions may play some role in the pathogenesis of pulmonary lymphoproliferative disorders. The frequency of oligoclonal infiltrating T cell expansions in human immunodeficiency virus (HIV)-related LIP (100%) was significantly higher than in low-grade pulmonary MALT lymphomas (40%) or in HIV-negative LIP (30%). Because recent evidence demonstrates that the V3 loop in the proviral amino acid sequences of mononuclear cells from bronchoalveolar lavage is more homogeneous than those from peripheral blood, this homogeneity might result in oligoclonal expansions of infiltrating T lymphocytes as a consequence of ongoing reactions against lung-specific viral strains.