Genetically Engineered Methanotroph as a Platform for Bioaugmentation of Chemical Pesticide Contaminated Soil
Genetically Engineered Methanotroph as a Platform for Bioaugmentation of Chemical Pesticide Contaminated Soil
复制标题
基因工程甲烷氧化菌作为化学农药污染土壤生物强化的平台
DOI：
10.1021/acssynbio.0c00532
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发表时间：
2021-02-22
影响因子：
4.7
通讯作者：
Liu, Juan
中科院分区：
文献类型：
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作者：
Liu, Yongchuang;Zhang, Haili;Liu, Juan
生物强化是土壤修复中一种有前景的替代方法。生物强化面临的一个挑战是，添加到土壤中的外源污染物降解微生物无法建立足够的生物量来消除污染物。考虑到甲烷氧化菌在有甲烷存在的情况下具有生长优势，我们假设基因工程改造的甲烷氧化菌能够在有甲烷的土壤中高效降解污染物。在此，选择了甲烷氧化菌甲基单胞菌属LW13、除草剂苄嘧磺隆（BSM）以及两种土壤来验证这一假设。首先针对菌株LW13开发了无标记基因敲入方法。然后，将BSM水解酶编码基因sulE插入菌株LW13的染色体中，使其具备BSM降解能力。接种后，在提供甲烷的情况下，菌株LW13 - sulE在土壤中的细胞数量大幅增加（9天内增加超过100倍）；同时，土壤中超过90%的BSM被降解。这项研究为基因工程改造的甲烷氧化菌是土壤修复的一个潜在平台这一概念提供了证据。
Bioaugmentation is a promising alternative in soil remediation. One challenge of bioaugmentation is that exogenous pollutant-degrading microbes added to soil cannot establish enough biomass to eliminate pollutants. Considering that methanotrophs have a growth advantage in the presence of methane, we hypothesize that genetically engineered methanotrophs could degrade contaminants efficiently in soil with methane. Here, methanotroph Methylonionas sp. LW13, herbicide bensulfuron-methyl (BSM), and two kinds of soil were chosen to confirm this hypothesis. The unmarked gene knock-in method was first developed for strain LW13. Then, BSM hydrolase encoding gene sulE was inserted into the chromosome of strain LW13, conferring it BSM-degrading ability. After inoculation, the cell amount of strain LW13-sulE in soil raised considerably (over 100 fold in 9 days) with methane provision; meanwhile, >90% of BSM in soil was degraded. This study provides a proof of the concept that genetically engineered methanotroph is a potential platform for soil remediation.