DNA INJECTION DURING BACTERIOPHAGE-T4 INFECTION OF ESCHERICHIA-COLI
DNA INJECTION DURING BACTERIOPHAGE-T4 INFECTION OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.154.2.938-945.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
MIZUSHIMA, S
中科院分区：
文献类型：
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作者：
FURUKAWA, H;KUROIWA, T;MIZUSHIMA, S
用4 ″，6-二脒基-2-苯基吲哚作为DNA特异性荧光染料，在荧光显微镜下研究了噬菌体T_4 DNA注入宿主细胞的过程。当用具有突出核心的人工收缩的噬菌体颗粒感染原生质球时，观察到噬菌体DNA注射。DNA注射是由核心尖端与原生质体细胞质膜的相互作用介导的。DNA注射过程不需要膜电位。DNA注射后感染的完整的非收缩噬菌体的完整的宿主细胞被抑制的能量毒药。基于这些观察，再加上从以前的工作的结果，T4感染过程的模型，并在感染过程中的膜电位的作用进行了讨论。
The process of phage T4 DNA injection into the host cell was studied under a fluorescent microscope, using 4'',6-diamidino-2-phenylindole as a DNA-specific fluorochrome. The phage DNA injection was observed when spheroplasts were infected with the artificially contracted phage particles having a protruding core. The DNA injection was mediated by the interaction of the core tip with the cytoplasmic membrane of the spheroplast. A membrane potential was not required for the process of DNA injection. DNA injection upon infection by intact noncontracted phage of the intact host cell was inhibited by an energy poison. Based on these observations, together with results from previous work, a model for the T4 infection process is presented, and the role of the membrane potential in the infection process is discussed.