Expression of MHC class II DQ alpha/beta heterodimers from recombinant polycistronic retroviral genomes.
Expression of MHC class II DQ alpha/beta heterodimers from recombinant polycistronic retroviral genomes.
复制标题
来自重组多顺反子逆转录病毒基因组的 MHC II 类 DQ α/β 异二聚体的表达。
DOI：
10.1007/s005950300041
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
LeGuern,Christian
中科院分区：
文献类型：
--
作者：
Shimada,Hideaki;Germana,Sharon;Hayashi,Hideki;Sachs,DavidH;LeGuern,Christian
Purpose:In the swine model, the transfer of the polymorphic DRβ or DQα/β cDNAs in vivo via double copy retroviruses led to a prolonged survival or tolerance of subsequent kidney grafts which were matched for DR or DQ, respectively. However, DQ-induced tolerance required the expression of the α/β heterodimers in the same target cell, a task not reproducibly achieved with double copy vectors. Therefore, the present study was designed to evaluate the ability of polycistronic proviral constructs to express class II DQ α/β heterodimers in transduced cells.Methods:A swine class II DQ recombinant polycistronic construct (JAB) was developed to contain two internal ribosomal entry sites (IRES) for sequential translation of the DQα and DQβ chains.Results:Although a genomic recombination occurred between the two identical IRES, flow cytometry analyses of JAB-transfected virus-packaging cells demonstrated the cell surface expression of DQα/DQβ heterodimers, indicative of a correct transcription, translation, and transport of swine class II.Conclusion:JAB-transfected virus-packaging cells demonstrated the cell surface expression of DQα/DQβ heterodimers. We believe that our study represents an essential step in the design of efficient protocols to transfer graft-matched class II molecules in recipient bone marrow cells and thereby induce transplantation tolerance.
