Application of Ultrasound-Ultrafiltration-Assisted alkaline isoelectric precipitation (UUAAIP) technique for producing alfalfa protein isolate for human consumption: Optimization, comparison, physicochemical, and functional properties
Application of Ultrasound-Ultrafiltration-Assisted alkaline isoelectric precipitation (UUAAIP) technique for producing alfalfa protein isolate for human consumption: Optimization, comparison, physicochemical, and functional properties
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DOI：
10.1016/j.foodres.2019.108907
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发表时间：
2020-04-01
影响因子：
8.1
通讯作者：
Ibarz, Albert
中科院分区：
文献类型：
--
作者：
Hadidi, Milad;Khaksar, Fatemeh Baradaran;Ibarz, Albert
采用超声-超滤辅助碱性等电沉淀法(UUAAIP)从紫花苜蓿叶片中提取紫花苜蓿分离蛋白。采用五因素三水平Box-Behnken设计(BBD)对UUAAIP工艺进行优化，使提取得率和蛋白质含量同时最大化。在液固比为43.3mL/g，pH为10.1，提取温度为42.5℃，提取时间为102min，L流量为9.7g/h的条件下，最大提取得率为14.5g/100g，蛋白质含量为91.1g/100g。UUAAIP技术提高了分离物的平均相对分子质量和色泽。此外，与热凝提取法(HCE)和碱性等电沉淀法(AIPE)相比，它能有效地去除抗营养因子中的皂苷和酚类化合物。与HCE和AIPE相比，UUAAIP提取的分离蛋白具有更高的溶解性、持水性和吸油性，但乳化和起泡性能较差。
A new Ultrasound-Ultrafiltration-Assisted Alkaline Isoelectric Precipitation (UUAAIP) technique was employed for producing alfalfa protein isolate from alfalfa leaves. A five-factor-three-level Box-Behnken Design (BBD) was used to optimize the UUAAIP process for maximizing both extraction yield and protein content simultaneously. The optimum conditions were as follows: solvent/solid material ratio of 43.3 mL/g, pH of 10.1, extraction temperature of 42.5 degrees C, extraction time of 102 min, and flow rate of 9.7 L/h. Under these conditions, the maximum predicted extraction yield and protein content were 14.5 g/100 g and 91.1 g/100 g, respectively. UUAAIP technique improved the average molecular weight and color of the isolate. Moreover, it was efficient in removing the saponins and phenolic compounds as anti-nutritional factors compared to heat-coagulation extraction (HCE) and alkaline isoelectric precipitation extraction (AIPE). The protein isolate extracted by UUAAIP had greater solubility, water-holding and oil-binding capacities but lower emulsifying and foaming properties than those extracted by HCE and AIPE.