Identification and analysis of altered α1,6-fucosylated glycoproteins associated with hepatocellular carcinoma metastasis
Identification and analysis of altered α1,6-fucosylated glycoproteins associated with hepatocellular carcinoma metastasis
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DOI：
10.1002/pmic.200500707
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发表时间：
2006-11-01
期刊：
影响因子：
3.4
通讯作者：
Tang, Zhao-You
中科院分区：
文献类型：
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作者：
Dai, Zhi;Liu, Yin-Kun;Tang, Zhao-You
肿瘤转移可能与细胞糖蛋白的表达水平及其糖链部分的改变有关。为筛选与肝细胞癌（HCC）转移相关的异常α 1，6-岩藻糖基化糖蛋白，建立了一种高通量的糖组学方法，该方法包括双向电泳、蛋白质电子转移、凝集素亲和印迹和沉淀以及基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS/MS）。比较和分析了高转移和低转移肝癌细胞系的透镜小扁豆凝集素（LCA）亲和糖蛋白谱。在34种鉴定的糖蛋白中，有7种差异显示，它们是细胞角蛋白8（CK 8）、膜联蛋白I、膜联蛋白11、异质核核糖核蛋白A/B、PDZ和LIM结构域1、RNA结合基序蛋白4和poly（rC）结合蛋白1。与Hep 3B相比，CK 8与蓖麻凝集素1（RCA-1）和LCA具有更高的亲和力，而annexin I与LCA和Con A具有更高的亲和力。Western blot和免疫荧光分析显示，在高转移肝癌细胞系中CK 8、annexin I和annexin II表达上调。提示CK 8、annexin I和annexin II的表达水平和糖链结构的改变可能与肝癌的转移能力有关，并在肝癌的转移过程中起重要作用。
Tumor metastasis might be associated with the expression levels of cellular glycoproteins and the alteration of their glycan parts. In order to screen the aberrantly alpha 1,6-fucosylated glycoproteins related to hepatocellular carcinoma (HCC) metastasis, a high-throughput glycomic approach which consisted of 2-DE, electronic transfer of proteins, lectin affinity blot and precipitation, and MALDI-TOF-MS/MS, was established. Lens culinaris agglutinin (LCA) affinity glycoprotein profiles of higher and lower metastatic HCC cell lines were compared and analyzed. Seven out of 34 identified glycoproteins were differentially displayed; they were cytokeratin 8 (CK8), annexin I, annexin 11, heterogeneous nuclear ribonucleoprotein A/B, PDZ and LIM domain 1, RNA-binding motif protein 4, and poly(rC)-binding protein 1. On comparison with Hep3B, CK8 showed a higher affinity to Ricinus communis agglutinin 1 (RCA-1) and LCA, and annexin I presented a higher affinity to LCA and Con A by the lectin-binding assay. Furthermore, the up-regulation of CK8, annexin I, and annexin II were found by Western blot and immunofluorescence analysis in higher metastatic HCC cell lines. This implied that the alteration of CK8, annexin I, and annexin II both in their expression levels and their glycan parts might be related to metastatic ability, and play a critical role in the process of HCC metastasis.