Mouse anesthesia and analgesia.
Mouse anesthesia and analgesia.
复制标题
DOI：
10.1002/9780470942390.mo140179
复制
发表时间：
2015-03-02
影响因子：
--
通讯作者：
Pacharinsak, Cholawat
中科院分区：
文献类型：
--
作者：
Adams, Sean;Pacharinsak, Cholawat
在实验室环境中，为小鼠受试者提供麻醉和镇痛是一种常见且至关重要的做法。这些实践对于执行侵入性手术是必要的，可以长期固定敏感成像方式（例如，磁共振成像），并提供了内部和后心理疼痛的缓解。除了促进研究人员执行的程序外，麻醉和镇痛的提供对于保存动物福利以及对研究中使用的动物的人道处理至关重要。此外，麻醉和镇痛是由机构动物护理和使用委员会回顾的动物使用方案的重要组成部分，需要仔细考虑和计划特定动物模型。在本文中，我们为研究人员提供了技术大纲，该研究人员涵盖了通过两种途径（可注射和吸入剂）提供麻醉的指南，监测麻醉的指南，当前识别疼痛的技术以及用于治疗预防性镇痛的考虑因素。
Providing anesthesia and analgesia for mouse subjects is a common and critical practice in the laboratory setting. These practices are necessary for performing invasive procedures, achieving prolonged immobility for sensitive imaging modalities (magnetic resonance imaging for instance), and providing intra- and post-procedural pain relief. In addition to facilitating the procedures performed by the investigator, the provision of anesthesia and analgesia is crucial for the preservation of animal welfare and for humane treatment of animals used in research. Furthermore, anesthesia and analgesia are important components of animal use protocols reviewed by Institutional Animal Care and Use Committees, requiring careful consideration and planning for the particular animal model. In this article, we provide technical outlines for the investigator covering the provision of anesthesia by two routes (injectable and inhalant), guidelines for monitoring anesthesia, current techniques for recognition of pain, and considerations for administering preventative analgesia.