Canine CD20 gene
Canine CD20 gene
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DOI：
10.1016/j.vetimm.2005.05.011
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发表时间：
2005-12-15
影响因子：
1.8
通讯作者：
Hasegawa, A
中科院分区：
文献类型：
--
作者：
Kano, R;Inoiue, C;Hasegawa, A
人CD20抗原是一种35 kDa的细胞表面非糖基化疏水磷蛋白，在几乎所有人b细胞上一致表达，其单克隆抗体用于治疗人b细胞淋巴瘤。本研究克隆和测序了犬CD20基因，并研究了CD20 mRNA在犬外周血单个核细胞（PBMCs）、健康犬淋巴结和犬淋巴瘤细胞中的表达。以犬cDNA为模板，采用5′-RACE和3′-RACE方法对犬CD20基因进行了全长测序。犬CD20的cDNA序列全长1239bp，编码297个氨基酸。犬CD20的氨基酸序列与人类和小鼠的相似度分别为73%和68%。预测犬CD20与人类CD20一样，含有2个胞外结构域（EM）、4个跨膜结构域（TM）和3个胞内结构域（IC）的氨基酸序列域。RT-PCR法检测到犬CD20 mRNA在健康犬外周血、淋巴结和犬淋巴瘤b细胞中表达，但在t细胞淋巴瘤细胞、非t细胞和非b细胞淋巴瘤细胞中不表达。基于这些结果，犬CD20可能成为犬b细胞淋巴瘤单克隆抗体治疗的靶点。(c) 2005 Elsevier B.V.版权所有
The human CD20 antigen, a 35 kDa cell surface nonglycosylated hydrophobic phoshpoprotein is expressed consistently on almost all human B-cells, and its monoclonal antibody is used for the therapy on human B-cell lymphoma. In the present study, canine CD20 gene was cloned and sequenced, and the expression of CD20 mRNA was investigated in canine peripheral blood mononuclear cells (PBMCs), and lymph nodes from healthy dogs, and canine lymphoma cells. Using canine cDNA as a template, full-length of canine CD20 gene was sequenced by 5'-RACE and 3'-RACE methods. The full-length of the cDNA sequence of canine CD20 was 1239 bp encoding 297 amino acids. The amino acid sequences of canine CD20 showed 73 and 68% sequence similarities with those of human and mouse, respectively. Canine CD20 was predicted to contain domains of amino acid sequences consisting of two extracellular domains (EM), four transmembrane domains (TM), and three intracellular domains (IC) as in human CD20. Canine CD20 mRNA was detected in PBMCs and lymph node from healthy dogs, and B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells by RT-PCR analysis. From these results, canine CD20 might be targeted for monoclonal antibody therapy against B-cell lymphoma of dogs. (c) 2005 Elsevier B.V. All rights reserved.