Long-Term Potentiation in the Neonatal Rat Barrel Cortex In Vivo
Long-Term Potentiation in the Neonatal Rat Barrel Cortex In Vivo
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DOI：
10.1523/jneurosci.1212-12.2012
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发表时间：
2012-07-11
影响因子：
5.3
通讯作者：
Luhmann, Heiko J.
中科院分区：
文献类型：
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作者：
An, Shuming;Yang, Jenq-Wei;Luhmann, Heiko J.
长时程增强（LTP）对于早期皮层回路的活动依赖性形成是重要的。在新生啮齿类动物桶皮质，LTP已被研究仅在体外。我们结合电压敏感染料成像与细胞外多电极记录，以研究在体内新生大鼠桶皮质的胡须-桶皮质通路中胡须刺激诱导的LTP。以2 Hz频率刺激单根胡须10 min，可诱导出生后0 - 14天大鼠的LTP表达随年龄增长而增加，其中P3-P5时LTP表达最强。LTP的幅度最大的激活桶相关的柱，较小的周围隔区，并没有LTP可以观察到在相邻桶。电流源密度分析显示，LTP相关的增加突触电流汇在第IV层/下层II/III在P3-P5和在皮质板/上层V在P0-P1。我们的研究表明，第一次在新生大鼠的桶皮质在体内的年龄依赖性和空间局限的LTP。
Long-term potentiation (LTP) is important for the activity-dependent formation of early cortical circuits. In the neonatal rodent barrel cortex, LTP has been studied only in vitro. We combined voltage-sensitive dye imaging with extracellular multielectrode recordings to study whisker stimulation-induced LTP in the whisker-to-barrel cortex pathway of the neonatal rat barrel cortex in vivo. Single whisker stimulation at 2 Hz for 10 min induced an age-dependent expression of LTP in postnatal day (P) 0 to P14 rats, with the strongest expression of LTP at P3-P5. The magnitude of LTP was largest in the activated barrel-related column, smaller in the surrounding septal region, and no LTP could be observed in the neighboring barrel. Current source density analyses revealed an LTP-associated increase of synaptic current sinks in layer IV/lower layer II/III at P3-P5 and in the cortical plate/upper layer V at P0-P1. Our study demonstrates for the first time an age-dependent and spatially confined LTP in the barrel cortex of the newborn rat in vivo.