Revitalization of frozen autologous bone graft using adipose-derived stem cells
Revitalization of frozen autologous bone graft using adipose-derived stem cells
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DOI：
10.15761/jts.1000329
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发表时间：
2020
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通讯作者：
Y. Aoki;N. Yamamoto;K. Hayashi;N. Sugimoto;H. Kimura;A. Takeuchi;S. Miwa;K. Igarashi;H. Inatani;T. Higuchi;Kensaku Abe;Yuta Taniguchi;Yoshihiro Araki;H. Tsuchiya
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作者：
Y. Aoki;N. Yamamoto;K. Hayashi;N. Sugimoto;H. Kimura;A. Takeuchi;S. Miwa;K. Igarashi;H. Inatani;T. Higuchi;Kensaku Abe;Yuta Taniguchi;Yoshihiro Araki;H. Tsuchiya
恶性骨肿瘤切除后存在各种重建方法，包括自体移植物的再循环，例如高压灭菌、巴斯德或辐照的自体移植物。自1999年以来，我们开发并临床应用了一种新的生物重建方法，使用液氮处理的带肿瘤自体移植物[1]。除了自体移植物的再循环，例如简单和完美的配合，没有病原体的繁殖，或没有过敏反应，液氮的使用呈现出几个优点。它具有良好的骨诱导和骨传导潜力，易于附着软组织和冷冻免疫，并提供足够的骨量和机械硬度[1-4]。然而，冷冻骨是肿瘤切除后骨缺损生物重建的有用替代方案，因为移植后骨的再生需要很长时间。延迟骨愈合是生物重建病例的主要问题，冷冻自体移植物的使用频率低于高压灭菌、辐照和巴斯德自体移植物。我们报告了在使用冷冻自体移植物的长期结局中，术后平均6.2个月观察到宿主骨-冷冻骨愈合[5]。然而，在使用冷冻自体移植物后，我们经历了一些延迟的骨愈合，其中一些病例需要第二次手术。我们的目的是实现骨再生和改善宿主骨冷冻骨愈合，以克服延迟骨愈合。例如，如果我们在骨肿瘤切除术中能够保留病变对侧皮质，我们在某些情况下使用半皮质切除方法来保留更多的宿主骨。此外，我们开发了一种新的重建技术，使用椎弓根冷冻法。这些技术允许自体移植物通过血液获得更大量的蛋白质[6，7]。需要进一步的治疗方法来使骨骼快速再生，我们寻求一种非技术性的方法来促进再生。* 通信：Norio Yamamoto，Department of Orthopathic Surgery，Graduate School of Medical Sciences，金泽大学，13-1 Takaramachi，金泽，日本，电子邮件：norinori@med.kanazawa-u.ac.jp
Various methods exist for reconstruction after resection of malignant bone tumor, including recycling of the autograft, for example autoclaved, Pasteur, or irradiated autografts. Since 1999, we developed and have clinically applied a novel method of biological reconstruction using tumor-bearing autografts treated with liquid nitrogen [1]. Apart from autograft recycling, for example for simplicity and perfect fit, no propagation of pathogens, or no allergic reaction, the use of liquid nitrogen presents several advantages. It has good osteoinduction and osteoconduction potential, allows for easy attachment of soft tissue and cryoimmunology, and provides sufficient bone stock and mechanical hardness [1-4]. However, frozen bone is a useful alternative for biological reconstruction of bone defects post-tumor resection, as revitalization of bone following a transplant requires a long time. Delayed bone union was the main problem in biological reconstruction cases, and frozen autograft is used less often than autoclaved, irradiated, and Pasteur autografts. We reported a host bone–frozen bone union seen at a mean of 6.2 months postoperatively among the long-term outcomes of using frozen autografts [5]. However, we experienced some delayed bone unions after using frozen autograft, and some of these cases required a second surgery. We aimed to achieve bone revitalization and improvement of a host bone–frozen bone union to overcome delayed bone union. For example, we used a hemicortical resection method to preserve more host bone in some cases if we could preserve the contralateral cortex of the lesion during bone tumor resection. Furthermore, we developed a new technique of reconstruction using the pedicle freezing method. These techniques allowed the autografts to obtain a greater amount of protein through blood [6,7]. Further treatments that would allow rapid bone revitalization are desirable, and we sought a nontechnological method to promote revitalization *Correspondence to: Norio Yamamoto, Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takaramachi, Kanazawa, Japan, E-mail: norinori@med.kanazawa-u.ac.jp