ANTIGEN-PRESENTING CELLS IN THE FEMALE REPRODUCTIVE-TRACT - INFLUENCE OF THE ESTROUS-CYCLE ON ANTIGEN PRESENTATION BY UTERINE EPITHELIAL AND STROMAL CELLS
ANTIGEN-PRESENTING CELLS IN THE FEMALE REPRODUCTIVE-TRACT - INFLUENCE OF THE ESTROUS-CYCLE ON ANTIGEN PRESENTATION BY UTERINE EPITHELIAL AND STROMAL CELLS
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DOI：
10.1210/en.136.10.4526
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发表时间：
1995-10-01
期刊：
影响因子：
4.8
通讯作者：
ROSSOLL, RM
中科院分区：
文献类型：
--
作者：
WIRA, CR;ROSSOLL, RM
本研究的目的是确定女性生殖道粘膜免疫系统的传入臂。当子宫细胞与卵清蛋白特异性T细胞和卵清蛋白孵育时，测量纯化的管腔上皮细胞和基质细胞的抗原呈递。完整大鼠的子宫细胞在整个生殖周期的分析表明，抗原呈递是由雌性激素控制。上皮细胞的抗原呈递在动情间期（第3天）高，此时雌二醇水平升高，而在动情期和动情间期（第1天和第2天）低。相反，基质细胞的抗原呈递在动情间期（第3天）较低，在动情期和动情间期（第1天和第2天）较高。与生理盐水对照组相比，给予卵巢切除大鼠的雌二醇刺激上皮细胞并抑制基质细胞抗原呈递。为了确定子宫细胞是否与其他细胞相互作用以促进抗原呈递，将胸腺树突样细胞与子宫上皮细胞一起孵育。我们的研究结果表明，抗原提呈协同增强，表明上皮细胞可以与基质抗原提呈细胞相互作用。抗体中和研究表明，上皮细胞和基质细胞抗原呈递都是通过II类细胞间粘附分子-1和淋巴细胞功能相关分子-1 α和-1 β介导的，它们起辅助分子的作用。这些研究表明，子宫上皮细胞和基质细胞对抗原的攻击有反应，性激素在调节子宫细胞的抗原呈递中起核心作用。
The objective of the present study was to define the afferent arm of the mucosal immune system in the female reproductive tract. When uterine cells were incubated with ovalbumin-specific T cells and ovalbumin, antigen presentation by purified luminal epithelial cells and stromal cells was measured. Analysis of uterine cells from intact rats throughout the reproductive cycle indicated that antigen presentation is controlled by the female sex hormones. Antigen presentation by epithelial cells was high at diestrus (day 3), when estradiol levels are elevated, and low at estrus and diestrus (days 1 and 2). In contrast, antigen presentation by stromal cells was low at diestrus (day 3) and high at estrus and diestrus (days 1 and 2). Estradiol given to ovariectomized rats stimulated epithelial cell and inhibited stromal cell antigen presentation compared with saline controls. To determine whether uterine cells interact with other cells to facilitate antigen presentation, thymus dendritic-like cells were incubated with uterine epithelial cells. Our findings indicated that antigen presentation is enhanced synergistically, suggesting that epithelial cells can interact with stromal antigen-presenting cells. Antibody neutralization studies indicated that both epithelial and stromal cell antigen presentation is mediated through class II, intercellular adhesion molecule-1 and lymphocyte function-associated molecules-1 alpha and -1 beta, which function as accessory molecules. These studies demonstrate that uterine epithelial and stromal cells are responsive to antigenic challenge and that sex hormones play a central role in regulating antigen presentation by uterine cells.