A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers
A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers
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DOI：
10.1016/s0379-0738(02)00195-0
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发表时间：
2002-09-10
影响因子：
2.2
通讯作者：
Hammer, MF
中科院分区：
文献类型：
--
作者：
Butler, JM;Schoske, R;Hammer, MF
建立了一种能同时扩增20个Y染色体短串联重复序列(STR)标记的多重聚合酶链式反应(PCR)分析方法，用于辅助人类身份鉴定和男性群体研究。这些标记包括构成欧洲使用的“扩展单倍型”的所有Y STR(DYS19、DYS385、DYS3891/II、DYS390、DYS391、DYS392、DYS393和YCAII)以及额外的多态Y STR(DYS437、DYS438、DYS439、DYS447、DYS448、DYS388、DYS426、GATA A7.1和GATA H4)。标记DYS385、DYS389和YCAII的引物针对Y染色体的重复区域，因此可以为每个相应的引物组提供两个多态峰。该Y-STR 20plex使用了34种不同的聚合酶链式反应引物，首次同时扩增了欧洲“最小”和“扩展”单倍型中的所有标记。将新开发的引物与以前发表的引物的相对位置进行比较。在设计引物时，尽量避免X染色体的同源性，并通过仔细检查已知的等位基因范围，将所有等位基因的大小保持在350bp以下。20plex与商业试剂盒之间的单倍型比较发现，Y染色体联盟小组的76个样本之间有很好的一致性。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
A multiplex polymerase chain reaction (PCR) assay capable of simultaneously amplifying 20 Y chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the "extended haplotype" used in Europe (DYS19, DYS385, DYS3891/II, DYS390, DYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs (DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYS426, GATA A7.1, and GATA H4). Primers for the markers DYS385, DYS389, and YCAII target duplicated regions of the Y chromosome and thus can provide two polymorphic peaks for each respective primer set. This Y STR 20plex, which utilizes 34 different PCR primers, is the first to include a simultaneous amplification of all the markers within the European "minimal" and "extended" haplotypes. Relative primer positions are compared between the newly developed primers described here and previously published ones. Efforts were made to avoid X chromosome homology in the primer design as well as close packing of PCR product size ranges in order to keep all alleles less than 350 bp through careful examination of known allele ranges. Haplotype comparisons between the 20plex and a commercially available kit found excellent agreement across the 76 samples in the Y chromosome consortium panel. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.