Identification of premycorrhiza-related plant genes in the association between Quercus robur and Piloderma croceum
Identification of premycorrhiza-related plant genes in the association between Quercus robur and Piloderma croceum
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DOI：
10.1111/j.1469-8137.2004.01091.x
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发表时间：
2004-07-01
期刊：
影响因子：
9.4
通讯作者：
Oelmüller, R
中科院分区：
文献类型：
--
作者：
Krüger, A;Peskan-Berghöfer, T;Oelmüller, R
用微繁殖的橡树（Quercus robur）和外生菌根真菌Pillacroceum（其特征在于菌根形成延迟）的体外系统来鉴定在前菌根阶段上调的植物转录本。通过反向北方证实了差异表达，并且发现不同多肽的50个克隆被上调。对29个克隆进行了更详细的研究，大约一半的cDNA片段在数据库中没有同源性。剩余片段编码的多肽与已知的蛋白质的同源性参与信号的感知和传输，应激反应，代谢和growth.As许多已确定的基因尚未被描述的共生事件的背景下，他们的潜在作用在早期阶段的识别过程中进行了讨论。
An in vitro system with micropropagated oaks (Quercus robur) and the ectomycorrhizal fungus Piloderma croceum, which is characterized by a delayed mycorrhiza formation, was used to identify plant transcripts upregulated in the premycorrhizal phase.Complementary DNA (cDNA) populations of uninoculated roots and fungal mycelium were subtracted from a cDNA population of inoculated roots. Differential expression was confirmed by reverse Northern and 50 clones for different polypeptides were found to be up-regulated. Twenty-nine clones were investigated in more detail.For approximately half of the cDNA fragments no homologies could be identified in databases. The residual fragments code for polypeptides with homologies to known proteins involved in signal perception and transmission, stress responses, metabolism and growth.Since many of the identified genes have not yet been described in the context of symbiotic events, their potential roles during early phases of the recognition process are discussed.