Binding and phagocytosis of fibronectin-coated beads by BHK cells: receptor specificity and dynamics.
Binding and phagocytosis of fibronectin-coated beads by BHK cells: receptor specificity and dynamics.
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BHK 细胞对纤连蛋白包被珠的结合和吞噬作用：受体特异性和动力学。
DOI：
10.1002/jcp.1041240211
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发表时间：
1985
影响因子：
5.6
通讯作者：
Grinnell,F
中科院分区：
文献类型：
--
作者：
McAbee,DD;Grinnell,F
对成纤维细胞吞噬乳胶珠的受体特异性和动力学的研究表明：（1）当包被在乳胶珠上时，发现除了纤维连接蛋白以外的配体，如刀豆球蛋白A（ConA）和血清扩散因子，可以促进乳胶珠的吞噬。这表明成纤维细胞吞噬作用与扩散一样，是一种配体-受体介导的现象，不特异性需要纤连蛋白（pFN）;（2）发现抗pFN抗体抑制细胞摄取先前结合在细胞表面的pFN包被珠粒的能力。因此，珠粒与细胞表面的结合本身并不是促进珠粒摄入的充分信号;（3）最后，二价阳离子保护了pFN包被珠粒吞噬所必需的受体功能，使其免受胰蛋白酶的蛋白水解，正如先前在pFN包被培养皿上发现的参与细胞附着和扩散的受体一样。用表面受体被破坏的细胞进行的恢复实验表明，存在一个内部（或隐蔽的细胞表面）受体库，其数量至少为细胞表面上正常发现的受体的50%。在完全破坏细胞表面和受体的隐蔽池后，珠结合和吞噬作用所需的受体的再现需要几个小时，并且在没有新蛋白质合成的情况下不会发生。
Studies on the receptor specificity and dynamics involved in fibroblast phagocytosis of latex beads revealed the following: (1) Ligands other than fibronectin such as concanavalin A (ConA) and serum spreading factor, when coated on latex beads, were found to promote phagocytosis of the beads. This indicates that fibroblast phagocytosis, like spreading, is a ligand‐receptor mediated phenomenon not specifically requiring fibronectin (pFN); (2) Anti‐pFN antibodies were found to inhibit the ability of cells to ingest pFN‐coated beads that previously were bound on the cell surfaces. Consequently, binding of beads to the cell surfaces per se is not a sufficient signal to promote ingestion of the beads; (3) Finally, divalent cations protected receptor function necessary for phagocytosis of pFN‐coated beads from proteolysis by trypsin, as previously was found for receptors involved in cell attachment and spreading on pFN‐coated culture dishes. Recovery experiments carried out with cells whose surface receptors had been destroyed indicated that there was an internal (or cryptic cell surface) pool of receptors that amounted to at least 50% of the receptors normally found on the cell surface. After complete destruction of the cell surface and cryptic pools of receptors, reappearance of receptors required for bead binding and phagocytosis required several hours and did not occur in the absence of new protein synthesis.