In vivo studies support the role of trafficking and cytoskeletal-binding motifs in the interaction of MESA with the membrane skeleton of Plasmodium falciparum-infected red blood cells
In vivo studies support the role of trafficking and cytoskeletal-binding motifs in the interaction of MESA with the membrane skeleton of Plasmodium falciparum-infected red blood cells
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DOI：
10.1016/j.molbiopara.2008.04.001
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发表时间：
2008-08-01
影响因子：
1.5
通讯作者：
Coppel, Ross L.
中科院分区：
文献类型：
--
作者：
Black, Casilda G.;Proellocks, Nicholas I.;Coppel, Ross L.
在感染疟疾寄生虫恶性疟原虫的红细胞（RBC）中，成熟寄生虫感染的红细胞表面抗原（梅萨）的19个残基区域与RBC细胞骨架蛋白4.1 R相关;寄生虫生存所必需的相互作用。梅萨中的该区域与在输出到膜骨架的其他疟原虫蛋白中发现的宿主靶向基序相邻。为了证明这些基序在体内的功能，在疟疾寄生虫中表达与报告基因融合的梅萨区域。免疫化学分析证实了这两个图案的运输和相互作用的梅萨与细胞骨架的要求，并证明其在体内的功能。(C)2008 Elsevier B. V.保留所有权利。
In red blood cells (RBCs) infected with the malaria parasite Plasmodium falciparum, a 19-residue region of the mature parasite-infected erythrocyte surface antigen (MESA) associates with RBC cytoskeleton protein 4.1 R; an interaction essential for parasite survival. This region in MESA is adjacent to a host targeting motif found in other malaria parasite proteins exported to the membrane skeleton. To demonstrate function of these motifs in vivo, regions of MESA fused to a reporter were expressed in malaria parasites. Immuno-chemical analyses confirmed the requirement for both motifs in the trafficking and interaction of MESA with the cytoskeleton and demonstrates their function in vivo. (C) 2008 Elsevier B.V. All rights reserved.