Relation between cell membrane tension and repair of membrane damaged during sonoporation.
Relation between cell membrane tension and repair of membrane damaged during sonoporation.
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细胞膜张力与声孔过程中受损膜修复之间的关系。
DOI：
10.1109/ultsym.2013.0453
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kudo N.
中科院分区：
文献类型：
--
作者：
Tanaka Y;Kudo N.
我们一直在研究一种声穿孔技术，并报道了短脉冲超声的一次暴露可以在附着在细胞上的微泡存在的情况下引起声穿孔效应。一般认为，声穿孔对在刚性支架上培养的细胞造成的损伤比悬浮在培养基中的细胞或对体内组织细胞造成的损伤更严重。一种可能的解释是，较高的损伤率是由较高的膜张力引起的。由于细胞从膜损伤中拯救自己的能力强烈依赖于它们的膜张力，因此在刚性支架上培养的细胞具有较高的膜张力，可能具有更严重的声穿孔损伤。在这项研究中，通过改变培养基的渗透压和选择盖盖上培养的细胞形状来控制细胞膜张力，并通过荧光显微镜观察来研究膜张力对膜修复能力的影响，这种观察可以直观地显示声波诱导的细胞损伤的严重程度。等渗和高渗条件下的细胞修复率分别为55±8%和75±14%（平均值±sd），表明膜张力越低，修复率越高。用不同形状的细胞对修复能力进行了研究。结果表明，具有较低膜张力的球形细胞的最小致死损伤水平是梭形细胞的5倍，这也表明较低的膜张力可以提高细胞对超声诱导的膜损伤的修复能力。
We have been studying a sonoporation technique and have reported that one-shot exposure of short-pulsed ultrasound can elicit a sonoporation effect in the presence of a microbubble attached to a cell. It is generally thought that sonoporation causes more severe damage to cells cultured on a rigid scaffold than to cells suspended in a medium or to cells of in vivo tissue. One possible explanation is that a higher damage rate is caused by higher membrane tension. Since the ability of cells to rescue themselves from membrane damage is strongly dependent on their membrane tension, cells cultured on a rigid scaffold, which have higher membrane tension, may have more severe sonoporation damage. In this study, cell membrane tension was controlled by changing osmolality of the culture medium and selecting shapes of cells cultured on a coverslip, and the effect of membrane tension on ability for membrane repair was investigated by fluorescence microscopic observation that visualizes severity of sonoporation-induced cell damage. Repair rates of cells treated under isotonic and hypertonic conditions of osmolality were 55±8%and 75±14% (means ± S.D.), respectively, indicating that lower membrane tension causes higher repair rate. The repair ability was also studied using cells with different shapes. The results showed that spherical-shaped cells, which have lower membrane tension, have a minimum lethal damage level five-times higher than that of spindle-shaped cells, also suggesting that lower membrane tension can increase the ability of cells to repair sonoporation-induced membrane damage.