MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals.
MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals.
复制标题
DOI：
10.3390/metabo4020319
复制
发表时间：
2014-05-09
期刊：
影响因子：
4.1
通讯作者：
Miura D
中科院分区：
文献类型：
--
作者：
Fujimura Y;Miura D
了解生物活性小分子的空间分布对于阐明其生物学或药学作用是必不可少的。质谱成像（MSI）能够通过直接电离和检测来确定全身或单个异质器官样品的组织切片中存在的可电离分子的分布。这种新兴的技术现在被广泛用于内源性或外源性小分子的原位无标记分子成像。MSI允许同时可视化许多类型的分子，包括母体分子及其代谢物。因此，MSI作为病理分析、理解药物机制和生物标志物发现的潜在工具受到了广泛关注。另一方面，关于MSI的技术局限性的几个问题至今仍未解决。在这篇综述中，我们描述了最新的基质辅助激光解吸/电离（MALDI）-MSI技术用于可视化内源性代谢物或膳食植物化学物质原位代谢的能力（食品因素），并讨论了技术问题和新的挑战，包括MALDI基质选择和代谢物鉴定，需要解决的有效和广泛的应用MSI在生物，生物医学和营养保健品（食品功能性）研究。
Understanding the spatial distribution of bioactive small molecules is indispensable for elucidating their biological or pharmaceutical roles. Mass spectrometry imaging (MSI) enables determination of the distribution of ionizable molecules present in tissue sections of whole-body or single heterogeneous organ samples by direct ionization and detection. This emerging technique is now widely used for in situ label-free molecular imaging of endogenous or exogenous small molecules. MSI allows the simultaneous visualization of many types of molecules including a parent molecule and its metabolites. Thus, MSI has received much attention as a potential tool for pathological analysis, understanding pharmaceutical mechanisms, and biomarker discovery. On the other hand, several issues regarding the technical limitations of MSI are as of yet still unresolved. In this review, we describe the capabilities of the latest matrix-assisted laser desorption/ionization (MALDI)-MSI technology for visualizing in situ metabolism of endogenous metabolites or dietary phytochemicals (food factors), and also discuss the technical problems and new challenges, including MALDI matrix selection and metabolite identification, that need to be addressed for effective and widespread application of MSI in the diverse fields of biological, biomedical, and nutraceutical (food functionality) research.