RELATIVE ABUNDANCE OF SUBUNIT MESSENGER-RNAS DETERMINES GATING AND CA2+ PERMEABILITY OF AMPA RECEPTORS IN PRINCIPAL NEURONS AND INTERNEURONS IN RAT CNS
RELATIVE ABUNDANCE OF SUBUNIT MESSENGER-RNAS DETERMINES GATING AND CA2+ PERMEABILITY OF AMPA RECEPTORS IN PRINCIPAL NEURONS AND INTERNEURONS IN RAT CNS
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DOI：
10.1016/0896-6273(95)90076-4
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发表时间：
1995-07-01
期刊：
影响因子：
16.2
通讯作者：
MONYER, H
中科院分区：
文献类型：
--
作者：
GEIGER, JRP;MELCHER, T;MONYER, H
记录大鼠脑片单个细胞膜上谷氨酸激活电流，并结合RT-PCR介导的AMPA受体(AMPAR)亚单位mRNA分析。对AMPAR的分析；在海马和新皮质的主神经元和中间神经元，以及在听觉中继神经元和Bergmann神经胶质细胞中，表明GluR-B亚单位及其翻转版本决定了相对缓慢的门控受体的形成，而Glur-D亚单位促进了更快的门控受体的组装。AMPAR通道钙通透性与Glur-B相对丰度的关系与该亚基在决定天然受体钙通透性中的优势一致。结果表明，Glur-B和Glur-D亚单位基因的差异表达及其mRNAs的剪接和编辑是导致天然AMPAR通道门控和钙通透性差异的原因。
Recording of glutamate-activated currents in membrane patches was combined with RT-PCR-mediated AMPA receptor (AMPAR) subunit mRNA analysis in single identified cells of rat brain slices. Analysis of AMPARs; in principal neurons and interneurons of hippocampus and neocortex and in auditory relay neurons and Bergmann glial cells indicates that the GluR-B subunit tn its flip version determines formation of receptors with relatively slow gating, whereas the GluR-D subunit promotes assembly of more rapidly gated receptors. The relation between Ca2+ permeability of AMPAR channels and the relative GluR-B mRNA abundance is consistent with the dominance of this subunit in determining the Ca2+ permeability of native receptors. The results suggest that differential expression of GluR-B and GluR-D subunit genes, as well as splicing and editing of their mRNAs, account for the differences in gating and Ca2+ permeability of native AMPAR channels.