Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord
Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord
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DOI：
10.1242/dev.173807
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发表时间：
2019-06-01
期刊：
影响因子：
4.6
通讯作者：
Sagner, Andreas
中科院分区：
文献类型：
--
作者：
Delile, Julien;Rayon, Teresa;Sagner, Andreas
脊椎动物神经管中基因表达的协调时空调控决定了神经祖细胞的身份以及它们产生的神经元的功能和生理。已经取得了进展，破译基因调控程序，负责这一进程;然而，组织的复杂性阻碍了网络和基本机制的系统分析。为了解决这个问题，我们使用单细胞mRNA测序来分析胚胎9.5-13.5天之间发育中的小鼠神经管的颈部和胸部区域。我们证实，这些数据准确地概括了神经管的发育，使我们能够确定特定祖细胞和神经元群体的新标记。此外，该分析强调了以前未被充分认识的时间组成部分的机制，产生神经元的多样性，并揭示了共同的特点，在序列的转录事件，导致特定的神经元亚型的分化。总之，这些数据提供了对负责神经元特异性的机制的深入了解，并提供了用于分类脊髓细胞类型的基因表达纲要，这将支持未来对神经管发育，功能和疾病的研究。
The coordinated spatial and temporal regulation of gene expression in the vertebrate neural tube determines the identity of neural progenitors and the function and physiology of the neurons they generate. Progress has been made deciphering the gene regulatory programmes that are responsible for this process; however, the complexity of the tissue has hampered the systematic analysis of the network and the underlying mechanisms. To address this, we used single cell mRNA sequencing to profile cervical and thoracic regions of the developing mouse neural tube between embryonic days 9.5-13.5. We confirmed that the data accurately recapitulates neural tube development, allowing us to identify new markers for specific progenitor and neuronal populations. In addition, the analysis highlighted a previously underappreciated temporal component to the mechanisms that generate neuronal diversity, and revealed common features in the sequence of transcriptional events that lead to the differentiation of specific neuronal subtypes. Together, the data offer insight into the mechanisms that are responsible for neuronal specification and provide a compendium of gene expression for classifying spinal cord cell types that will support future studies of neural tube development, function and disease.