Characterization of the gene for Delta(1)-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L
Characterization of the gene for Delta(1)-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L
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DOI：
10.1023/a:1005702408601
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发表时间：
1997-03-01
影响因子：
5.1
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
从水稻（Oryza sativa cv. Akibare）14日龄幼苗制备的cDNA文库中分离并鉴定了编码Δ1 -吡咯啉 - 5 -羧酸（P5C）合成酶（cOsPSCS）的cDNA，该酶参与脯氨酸的生物合成。推导的水稻P5C合成酶蛋白（OsP5CS）的氨基酸序列与拟南芥（Arabidopsis thaliana）和乌头叶豇豆（Vigna aconitifolia）的P5C合成酶分别具有74.2%和75.5%的同源性。Northern杂交分析表明，P5C合成酶基因（OsP5CS）受高盐、脱水、脱落酸处理和低温处理诱导，但不受热处理诱导。同时，在水稻中观察到高盐处理导致脯氨酸积累。此外，比较了耐盐品种Dee - gee - woo - gen（DGWG）和盐敏感育种系IR28在盐胁迫条件下OsP5CS mRNA的表达水平和脯氨酸含量。观察到DGWG中P5C合成酶基因的表达和脯氨酸的积累稳定增加，而IR28中则略有增加。
A cDNA for Delta(1)-pyrroline-5-carboxylate (P5C) synthetase (cOsPSCS), an enzyme involved in the biosynthesis of proline, was isolated and characterized from a cDNA library prepared from 14-day-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the P5CS protein (OsP5CS) from O. sativa exhibited 74.2% and 75.5% homology to that of the P5CS from Arabidopsis thaliana and Vigna aconitifolia, respectively. Northern blot analysis revealed that the gene for P5CS (OsP5CS) was induced by high salt, dehydration, treatment of ABA and cold treatment, while it was not induced by heat treatment. Simultaneously, accumulation of proline was observed as a result of high salt treatment in O, sativa. Moreover, the levels of expression of OsP5CS mRNA and content of proline under salt stress condition were compared between a salt-tolerant cultivar, Dee-gee-woo-gen (DGWG) and a salt-sensitive breeding line, IR28. It was observed that the expression of the P5CS gene and the accumulation of proline in DGWG steadily increased, whereas those in IR28 increased slightly.