Immunologic and cytochemical cell markers in non-Hodgkin's lymphomas.
Immunologic and cytochemical cell markers in non-Hodgkin's lymphomas.
复制标题
非霍奇金淋巴瘤的免疫学和细胞化学细胞标记。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
A. Gottlieb
中科院分区：
文献类型：
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作者：
F. Davey;J. Goldberg;J. Stockman;A. Gottlieb
淋巴结来自28例非霍奇金淋巴瘤患者和24例非血液系统恶性肿瘤患者。分析了未分化淋巴瘤、弥漫性组织细胞淋巴瘤、弥漫性和结节性混合组织细胞淋巴瘤、结节性低分化淋巴细胞淋巴瘤和弥漫性高分化淋巴细胞淋巴瘤的病例。触摸准备染色非特异性酯酶，过氧化物酶，苏丹黑B活性和高碘酸希夫和赖特-吉姆萨试剂。检测淋巴结单核细胞悬液和（在某些情况下）外周血单核细胞悬液与绵羊红细胞的自发玫瑰花结形成以及表面免疫球蛋白的存在。用苏木精和伊红染色后检查淋巴结的其余部分。淋巴细胞表面标志分析表明，15例不同组织学类型的淋巴瘤均为B细胞增生。然而，弥漫性低分化淋巴细胞性淋巴瘤的4例中有3例和弥漫性组织细胞性淋巴瘤的7例中有1例表现为T细胞瘤形成。代表不同组织学类型的四例淋巴瘤的淋巴结被没有可辨别的细胞标志物的肿瘤细胞所取代。在5例病例中，恶性淋巴结中细胞表面标志物的分布与非肿瘤性淋巴结分析中获得的数据没有差异。该研究表明，目前采用的淋巴网状细胞恶性肿瘤分类中所识别的组织病理学实体是异质性的。12例患者中有5例外周血中细胞表面标志物分布的改变表明，4例患者外周血受累的明显形态学证据和2例患者骨髓浸润之前受累。
Lymph nodes were obtained from 28 patients with non-Hodgkin's lymphoma and 24 patients without hematologic malignancy. Cases of undifferentiated lymphoma, diffuse histiocytic lymphoma, diffuse and nodular mixed histiocytic-lymphocytic lymphoma, nodular poorly differentiated lymphocytic lymphoma, and diffuse well differentiated lymphocytic lymphoma were analyzed. Touch preparations were stained for nonspecific esterases, peroxidase, Sudan black B activity and with periodic acid-Schiff and Wright-Giemsa reagents. Mononuclear cell suspension from lymph nodes and, in some cases, peripheral blood were tested for spontaneous rosette formation with sheep erythrocytes and for the presence of surface immunoglobulin. The remainder of the lymph node was examined after staining with hematoxylin and eosin. Analysis of the lymphocyte surface markers indicated that 15 cases of various histologic types of lymphoma were B cell proliferations. However, three out of four cases of diffuse poorly differentiated lymphocytic lymphoma and one of seven cases of diffuse histiocytic lymphoma appeared to represent T cell neoplasia. Lymph nodes from four cases of lymphoma representing diverse histologic types were replaced by neoplastic cells devoid of discernible cell markers. In five cases, the distribution of cell surface markers in the malignant lymph node failed to differ from data obtained in the analysis of non-neoplastic lymph nodes. The study indicates that the histopathologic entities recognized in the currently employed classification of lymphoreticular malignancies are heterogeneous. Alterations in the distribution of cell surface markers in the peripheral blood from five of 12 patients indicated involvement prior to demonstrable morphologic evidence of peripheral blood involvement in four patients and bone marrow infiltration in two patients.