Tn2610, a transposon involved in the spread of the carbenicillin-hydrolyzing β-lactamase gene
Tn2610, a transposon involved in the spread of the carbenicillin-hydrolyzing β-lactamase gene
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Tn2610，一种参与羧苄青霉素水解β-内酰胺酶基因传播的转座子
DOI：
10.1007/bf00337818
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
T. Sawai
中科院分区：
文献类型：
--
作者：
Tomoko Yamamoto;Masato Watanabe;Kayoko Matsumoto;T. Sawai
我们在大肠杆菌临床分离株pCS 200上发现了一个新的转座子Tn 2610，它编码羧苄青霉素水解β-内酰胺酶基因，并与链霉素和磺胺的耐药决定簇结合。Tn 2610的分子大小为24个双链酶对，侧翼为3个双链酶对的长反向重复序列。遗传和物理分析表明，Tn 2610是一个单一的转座单位编码的多个抗性决定因素，这是不同于任何以前描述的转座子。本文讨论了参与羧苄青霉素水解β-内酰胺酶基因转座的各种复杂耐药转座子中观察到的特征性DNA结构。
We have found a new transposon, Tn2610, on pCS200 in clinical isolates ofEscherichia coli, which encodes the carbenicillin-hydrolyzing β-lactamase gene in combination with the resistance determinants to streptomycin and sulfonamide. Tn2610has a molecular size of 24 kilobase pairs and is flanked by long inverted repeat sequences of 3 kilobase pairs in length. Genetical and physical analyses indicate that Tn2610is a single transposable unit encoding the multiple resistance determinants and that is different from any previously described transposon. The characteristic DNA structure observed in various complex resistance transposons involved in the transposition of the carbenicillin-hydrolyzing β-lactamase gene is discussed.