An efficient method for hatching diapausing embryos of Daphnia pulex species complex (Crustacea, Anomopoda).
An efficient method for hatching diapausing embryos of Daphnia pulex species complex (Crustacea, Anomopoda).
复制标题
孵化水溞物种复合体(甲壳纲、无足纲)滞育胚胎的有效方法。
DOI:
10.1002/jez.2331
复制
发表时间:
2020
期刊:
影响因子:
--
通讯作者:
Xu,Sen
中科院分区:
文献类型:
--
作者:
Luu,DustinH-K;Vo,HillaryP;Xu,Sen
A general method for efficiently hatching sexually produced diapausing embryos of the microcrustaceanDaphniais valuable for establishingDaphniaas a genetic model system. In this study, we examine the effect of ultraviolet light and different amounts of storage time in darkness on the hatching efficiency in two species of theDaphnia pulexspecies complex,D. pulexandDaphnia pulicaria. We identified a set of lighting conditions that can trigger 80% to ~100% hatching rate for embryos produced through selfing, outcrossing, and obligate parthenogenesis. Furthermore, we found that a storage time of at least 2 weeks in the dark before exposing embryos to ultraviolet light is critical for achieving high hatching rate. The identification of these key factors for hatching diapausing embryos can greatly facilitate futureDaphniaresearch involving complex breeding designs as well as investigating the genetic switch that activates the hatching of diapausing embryos.
登录
查看更多内容
影响因子:
2.6
作者:
R. G. Stross
通讯作者:
R. G. Stross
影响因子:
1.6
作者:
Jacek Radzikowski;Katarzyna Krupińska;M. Ślusarczyk
通讯作者:
M. Ślusarczyk
DOI:
--
发表时间:
2016
期刊:
Brazilian journal of biology = Revista brasleira de biologia
影响因子:
--
作者:
T. A. Paes;A. C. Rietzler;P. Maia
通讯作者:
P. Maia
DOI:
--
发表时间:
1963
期刊:
影响因子:
--
作者:
John R. Pancella;R. G. Stross
通讯作者:
R. G. Stross
影响因子:
4.5
作者:
Kato Y;Kobayashi K;Watanabe H;Iguchi T
通讯作者:
Iguchi T