An efficient method for hatching diapausing embryos of Daphnia pulex species complex (Crustacea, Anomopoda).

An efficient method for hatching diapausing embryos of Daphnia pulex species complex (Crustacea, Anomopoda).
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孵化水溞物种复合体(甲壳纲、无足纲)滞育胚胎的有效方法。

DOI:
10.1002/jez.2331
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发表时间:
2020
期刊:
Journal of experimental zoology. Part A, Ecological and integrative physiology
影响因子:
--
通讯作者:
Xu,Sen
Xu,Sen
中科院分区:
--
文献类型:
--
作者:
Luu,DustinH-K;Vo,HillaryP;Xu,Sen

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一种有效孵化小甲壳纲水蚤有性繁殖滞育胚胎的通用方法,对水蚤遗传模型系统的建立具有重要意义。在本研究中,我们研究了紫外光和不同的黑暗储存时间对两种水蚤(daphnia pulexspecies complex,D.)的孵化效率的影响。pulexandDaphnia pulicaria。我们确定了一组光照条件,可以使通过自交、异交和专性孤雌生殖产生的胚胎的孵化率达到80% ~100%。此外,我们发现,在将胚胎暴露于紫外线光之前,至少在黑暗中保存2周对于获得高孵化率至关重要。这些滞育胚胎孵化的关键因素的确定,可以极大地促进未来涉及复杂育种设计的昆虫研究,以及研究激活滞育胚胎孵化的遗传开关。
A general method for efficiently hatching sexually produced diapausing embryos of the microcrustaceanDaphniais valuable for establishingDaphniaas a genetic model system. In this study, we examine the effect of ultraviolet light and different amounts of storage time in darkness on the hatching efficiency in two species of theDaphnia pulexspecies complex,D. pulexandDaphnia pulicaria. We identified a set of lighting conditions that can trigger 80% to ~100% hatching rate for embryos produced through selfing, outcrossing, and obligate parthenogenesis. Furthermore, we found that a storage time of at least 2 weeks in the dark before exposing embryos to ultraviolet light is critical for achieving high hatching rate. The identification of these key factors for hatching diapausing embryos can greatly facilitate futureDaphniaresearch involving complex breeding designs as well as investigating the genetic switch that activates the hatching of diapausing embryos.
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