Herpes simplex virus as a transneuronal tracer
Herpes simplex virus as a transneuronal tracer
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DOI：
10.1016/s0149-7634(98)00008-6
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发表时间：
1998-10-01
影响因子：
8.2
通讯作者：
Lehman, MN
中科院分区：
文献类型：
--
作者：
Norgren, RB;Lehman, MN
确定神经系统的连接对于确定它们的功能至关重要。在本综述中，我们重点介绍了HSV-1和HSV-2作为跨神经元示踪剂的应用。使用HSV检查神经回路在技术上很简单。将HSV注射到感兴趣的区域，几天后，对动物进行灌流，并用HSV蛋白抗体进行免疫组织化学处理。影响HSV感染的因素有宿主种类、宿主年龄、病毒滴度、病毒株和感染细胞表型。HSV毒株的选择是至关重要的，一些HSV-1和HSV-2毒株已被用于跨神经束追踪。HSV已被成功地用于研究各种不同的神经解剖系统中的神经元回路，包括躯体感觉、嗅觉、视觉、运动、自主神经和边缘系统。(C)1998爱思唯尔科学有限公司。保留所有权利。
Determining the connections of neural systems is critical for determining how they function. In this review, we focus on the use of HSV-1 and HSV-2 as transneuronal tracers. Using HSV to examine neural circuits is technically simple. HSV is injected into the area of interest, and after several days, the animals are perfused and processed for immunohistochemistry with antibodies to HSV proteins. Variables which influence HSV infection include species of host, age of host, titre of virus, strain of virus and phenotype of infected cell. The choice of strain of HSV is critically important Several strains of HSV-1 and HSV-2 have been utilized for purposes of transneuronal tract-tracing. HSV has been used successfully to study neuronal circuitry in a variety of different neuroanatomical systems including the somatosensory, olfactory, visual, motor, autonomic and limbic systems. (C) 1998 Elsevier Science Ltd. All rights reserved.