Specific stimulation of in vitro maturation of mesencephalic dopaminergic neurones by striatal membranes-猫眼课题宝

纹状体膜对中脑多巴胺能神经元体外成熟的特异性刺激

DOI：

发表时间：

1981

期刊：

Nature

影响因子：

64.8

通讯作者：

中科院分区：

综合性期刊1区

文献类型：

作者：

A. Prochiantz;M. Daguet;A. Herbet;J. Glowinski

我们以前已经建立了分离的中脑多巴胺能（DA）神经元从胚胎小鼠分化的原代培养物，他们的发展是增强时，他们的目标细胞从纹状体1 -3的存在下生长。单个DA神经元摄取和合成~ 3 H-DA的能力在共培养中增加。这种现象不依赖于神经胶质细胞的存在，因为它也发生在无血清条件下，其中神经胶质细胞的发育在很大程度上受到损害4。因此，纹状体靶神经元有利于传入DA神经元的成熟。扩散或膜结合因子可能参与这一过程。为了测试这些可能性之一，我们现在已经研究了胚胎中脑DA神经元暴露于纹状体膜分离的出生后小鼠在不同年龄的体外发育。3 H-DA摄取被用作DA神经元成熟的指标5。我们发现，纹状体膜从2-和3周龄的动物刺激DA神经元的发展。这一发现，这种影响是没有看到与膜从1周龄或成年动物表明，一个发育调节膜结合的“因素”可能参与。这个因素似乎是特定的DA神经元。

We have previously established that dissociated mesencephalic dopaminergic (DA) neurones from embryonic mouse differentiate in primary cultures and that their development is enhanced when they are grown in the presence of their target cells from the striatum1–3. The capacity of individual DA neurones to take up and synthesize 3H-DA was increased in co-culture. This phenomenon does not depend on the presence of glial cells because it also occurred in serum-free conditions in which glial development was largely impaired4. Therefore, striatal target neurones favour the maturation of afferent DA neurones. Diffusible or membrane-bound factors could be involved in this process. To test one of these possibilities, we have now examined the in vitro development of embryonic mesencephalic DA neurones exposed to striatal membranes isolated from postnatal mice at various ages. 3H-DA uptake was used as an index of maturation of the DA neurones5. We show that striatal membranes from 2- and 3-week-old animals stimulate the development of DA neurones. The finding that this effect was not seen with membranes from 1-week-old or adult animals suggests that a developmentally regulated membrane-bound ‘factor’is probably involved. This factor seems to be specific for the DA neurones.

Target neuron-specific process formation by embryonic mesencephalic dopamine neurons in vitro.