Chromosomal locus tracking with proper accounting of static and dynamic errors.
Chromosomal locus tracking with proper accounting of static and dynamic errors.
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DOI：
10.1103/physreve.91.062716
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Moerner WE
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文献类型：
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作者：
Backlund MP;Joyner R;Moerner WE
跟踪的单个粒子或分子的均方位移（MSD）和速度自相关（VAC）是用于提取描述对象运动的参数的普遍度量，但是它们都被实验误差破坏，这阻碍了基本参数的定量提取。对于纯布朗运动的简单情况，由于光子统计（“静态误差”）和运动模糊由于有限的曝光时间（“动态误差”）的MSD和VAC的本地化误差的影响已经被常规处理。然而，通过复杂的环境，如细胞，细胞核，或聚合物移动的粒子往往表现出异常的扩散，这些错误的影响往往是不够的治疗。我们目前的数据，从跟踪的染色体位点在酵母中，证明了适当占静态和动态误差的背景下，一个异常的扩散，这是一致的分数布朗运动（FBM）的必要性。我们将这些数据与存在这些误差的一般FBM的MSD和VAC的预期值的分析形式进行比较。
The mean-squared displacement (MSD) and velocity autocorrelation (VAC) of tracked single particles or molecules are ubiquitous metrics for extracting parameters that describe the object’s motion, but they are both corrupted by experimental errors that hinder the quantitative extraction of underlying parameters. For the simple case of pure Brownian motion, the effects of localization error due to photon statistics (“static error”) and motion blur due to finite exposure time (“dynamic error”) on the MSD and VAC are already routinely treated. However, particles moving through complex environments such as cells, nuclei, or polymers often exhibit anomalous diffusion, for which the effects of these errors are less often sufficiently treated. We present data from tracked chromosomal loci in yeast that demonstrate the necessity of properly accounting for both static and dynamic error in the context of an anomalous diffusion that is consistent with a fractional Brownian motion (FBM). We compare these data to analytical forms of the expected values of the MSD and VAC for a general FBM in the presence of these errors.