IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOTHELIAL NITRIC-OXIDE SYNTHASE IN HUMAN VILLOUS AND EXTRAVILLOUS TROPHOBLAST POPULATIONS AND EXPRESSION DURING SYNCYTIOTROPHOBLAST FORMATION IN-VITRO
IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOTHELIAL NITRIC-OXIDE SYNTHASE IN HUMAN VILLOUS AND EXTRAVILLOUS TROPHOBLAST POPULATIONS AND EXPRESSION DURING SYNCYTIOTROPHOBLAST FORMATION IN-VITRO
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DOI：
10.1016/0143-4004(95)90000-4
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发表时间：
1995-03-01
期刊：
影响因子：
3.8
通讯作者：
MYATT, L
中科院分区：
文献类型：
--
作者：
EIS, ALW;BROCKMAN, DE;MYATT, L
我们已经研究了分布的内皮型一氧化氮合酶（eNOS）在绒毛和绒毛外滋养细胞群体的免疫组化，并进一步研究了eNOS的表达在细胞滋养细胞分化成syncytiotrophoblast培养。在孕早期绒毛组织中，NADPH黄递酶活性和eNOS免疫染色存在于合体滋养层，但不祖细胞滋养层。抗细胞角蛋白免疫染色显示胎盘基板的绒毛外滋养细胞具有NADPH黄递酶活性，但eNOS免疫染色不显示。羊膜上皮细胞和绒毛膜细胞滋养层细胞均有NADPH黄递酶活性，但eNOS免疫染色阴性，而eNOS免疫染色见于羊膜成纤维细胞层。纯化的绒毛细胞滋养层细胞从长期胎盘聚集和融合，形成一个合胞体，随着培养时间的增加，抗桥粒蛋白和抗核抗体免疫染色评估。培养24 h后，大部分细胞仍为单核细胞滋养层细胞，未显示eNOS免疫染色，而少数合胞体聚集体形成，eNOS阳性和hPL阳性。培养3 - 5天后，大部分细胞以合体滋养层的形式存在。然而，eNOS和hPL的免疫染色是更广泛的，并不是所有的合胞体聚集阳性。在滋养层细胞中，只有合体滋养层细胞表达eNOS。细胞滋养层细胞向合体滋养层细胞的分化与eNOS表达相关。
We have examined the distribution of the endothelial isoform of nitric oxide synthase (eNOS) in villous and extravillous trophoblast populations by immunohistochemistry and have further studied expression of eNOS during differentiation of cytotrophoblast into syncytiotrophoblast in culture. In first trimester villous tissue, NADPH diaphorase activity and eNOS immunostaining were present in syncytiotrophoblast but not the progenitor cytotrophoblast layer. Extravillous trophoblast in the basal plate of the placenta was identified by anticytokeratin immunostaining and displayed NADPH diaphorase activity, but not eNOS immunostaining. Both amnion epithelial cells and chorion cytotrophoblast had NADPH diaphorase activity but no eNOS immunostaining, whereas eNOS immunostaining was seen in the fibroblast layer of amnion. Purified villous cytotrophoblast cells from term placentae aggregated and fused to form a syncytium with increasing time in culture as assessed by antidesmosomal protein and antinuclear antibody immunostaining. Following 24 h in culture, the majority, of cells were still mononucleate cytotrophoblast which did not display eNOS immunostaining, whereas a few syncytial aggregates had formed which were both eNOS positive and hPL positive. By 3 to 5 days in culture, the majority of cells were present as syncytiotrophoblast. However, eNOS and hPL immunostaining was more diffuse and not all syncytial aggregates were positive. Of the trophoblast populations, only syncytiotrophoblast appears to express eNOS. Differentiation of cytotrophoblast into syncytotrophoblast is associated with eNOS expression.