Phylogeny of North American Powassan virus
Phylogeny of North American Powassan virus
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DOI：
10.1099/0022-1317-82-7-1657
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发表时间：
2001-07-01
影响因子：
3.8
通讯作者：
Telford, SR
中科院分区：
文献类型：
--
作者：
Ebel, GD;Spielman, A;Telford, SR
为了确定是否Powassan病毒（POW）和鹿蜱病毒（DTV）构成不同的黄病毒种群传播的硬蜱在北美，我们分析了不同的核苷酸序列从16株这些病毒。两个不同的遗传谱系是明显的，这可能是由地理和主机协会定义。谱系1（包括纽约和加拿大POW分离株）的核苷酸和氨基酸序列在时间和空间上高度保守，但谱系2（包括鹿蜱和狐狸的分离株）的核苷酸和氨基酸序列变化更大。血统之间的分歧比任何一个血统内部的差异都要大得多，血统二似乎比血统一在遗传上更加多样化。McDonald-Kreitman测试对这些菌株的序列的应用表明，包膜蛋白的适应性进化将谱系1与谱系2分开。这两个POW谱系循环在北美拥有的遗传多样性的模式，这表明它们包括不同的亚型，可能会在单独的地方病周期中延续。
To determine whether Powassan virus (POW) and deer tick virus (DTV) constitute distinct flaviviral populations transmitted by ixodid ticks in North America, we analysed diverse nucleotide sequences from 16 strains of these viruses. Two distinct genetic lineages are evident, which may be defined by geographical and host associations. The nucleotide and amino acid sequences of lineage one (comprising New York and Canadian POW isolates) are highly conserved across time and space, but those of lineage two (comprising isolates from deer ticks and a fox) are more variable. The divergence between lineages is much greater than the variation within either lineage, and lineage two appears to be more diverse genetically than is lineage one. Application of McDonald-Kreitman tests to the sequences of these strains indicates that adaptive evolution of the envelope protein separates lineage one from lineage two. The two POW lineages circulating in North America possess a pattern of genetic diversity suggesting that they comprise distinct subtypes that may perpetuate in separate enzootic cycles.