Sanguinarine Induces Apoptosis of HT-29 Human Colon Cancer Cells via the Regulation of Bax/Bcl-2 Ratio and Caspase-9-Dependent Pathway
Sanguinarine Induces Apoptosis of HT-29 Human Colon Cancer Cells via the Regulation of Bax/Bcl-2 Ratio and Caspase-9-Dependent Pathway
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DOI：
10.1177/1091581811423845
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发表时间：
2012-01-01
影响因子：
2.2
通讯作者：
Kim, Hyung Keun
中科院分区：
文献类型：
--
作者：
Lee, Jun Sik;Jung, Won-Kyo;Kim, Hyung Keun
血根碱是从血根植物加拿大血根中提取的生物碱，对氧化应激和炎症性疾病具有有益作用。先前的报告已证明血根碱还具有抗癌特性。在当前的研究中，我们研究了血根碱对 HT-29 人结肠癌细胞的影响。据观察，血根碱治疗可诱导人结肠癌细胞凋亡的剂量依赖性增加。我们还研究了血根碱对凋亡相关蛋白表达的影响，结果显示 Bax 增加，B 细胞淋巴瘤 2 (Bcl-2) 蛋白水平降低。此外，血根碱治疗显着增加了半胱天冬酶 3 和 9 的激活，它们是细胞凋亡的关键执行者。我们的结果表明，血根碱可诱导 HT-29 人结肠癌细胞凋亡，并可能在治疗人结肠癌中具有潜在的治疗用途。
Sanguinarine is an alkaloid obtained from the bloodroot plant Sanguinaria canadensis and has beneficial effects on oxidative stress and inflammatory disorders. Previous reports have demonstrated that sanguinarine also exhibit anticancer properties. In the current study, we investigated the effects of sanguinarine on HT-29 human colon cancer cells. It was observed that sanguinarine treatment induces a dose-dependent increase in apoptosis of human colon cancer cells. We also investigated the effects of sanguinarine on the expression of apoptosis-associated proteins, and the results revealed that there was an increase in Bax and a decrease in B-cell lymphoma 2 (Bcl-2) protein levels. Moreover, sanguinarine treatment significantly increases the activation of caspases 3 and 9 that are the key executioners in apoptosis. Our results suggest that sanguinarine induces apoptosis of HT-29 human colon cancer cells and may have a potential therapeutic use in the treatment of human colon cancer.