Ethylene- and ozone-induced regulation of a grapevine resveratrol synthase gene: different responsive promoter regions
Ethylene- and ozone-induced regulation of a grapevine resveratrol synthase gene: different responsive promoter regions
复制标题
DOI：
10.1016/s0981-9428(02)01448-1
复制
发表时间：
2002-10-01
影响因子：
6.5
通讯作者：
Sandermann, H
中科院分区：
文献类型：
--
作者：
Grimmig, B;Gonzalez-Perez, MN;Sandermann, H
二苯乙烯脱氢酶（stilbene dismutase，STS）是在二苯乙烯类化合物的生物合成中起关键作用的酶，该化合物是由各种生物和非生物胁迫（如病原体攻击、UV辐射或臭氧暴露）诱导的植物抗毒素。为了研究臭氧和乙烯诱导的植物胁迫反应的分子基础，我们分析了葡萄藤藜芦醇合酶基因（Vst 1）的全长启动子。携带Vst 1启动子：β-葡萄糖醛酸糖苷酶（GUS）报道基因构建体的转基因烟草植物在臭氧（0.3穆尔升（-1），8小时）或乙烯（10穆尔升（-1），8小时）处理后显示出GUS活性增加。使用荧光GUS测定，5 '侧翼序列的缺失分析鉴定了翻译起始密码子上游-280和-40之间的最小乙烯响应区域。该区域不同于臭氧响应Vst 1启动子区域，表明乙烯不直接参与臭氧诱导的芪生物合成信号。(C)2002年，Elsevier SAS科学与医学版。All rights reserved.
Stilbene synthases (STSs) are enzymes that play a critical role in the biosynthesis of stilbenes, phytoalexins that are induced by various biotic and abiotic stressors like pathogen attack, UV radiation or ozone exposure. To investigate the molecular basis for ozone- and ethylene-induced plant stress responses, we analysed the full size promoter of the grapevine resveratrol synthase gene (Vst1). Transgenic tobacco plants harbouring a Vst1 promoter::beta-glucuronidase (GUS) reporter construct showed an increased GUS activity upon ozone (0.3 mul l(-1), 8 h) or ethylene (10 mul l(-1), 8 h) treatment. Using the fluorimetric GUS assay, deletion analysis of the 5'-flanking sequence identified a minimal ethylene-responsive region between -280 and -40 by upstream of the translation start codon. This region differs from the ozone-responsive Vst1 promoter region, suggesting that ethylene is not directly involved in signalling ozone-induced stilbene biosynthesis. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.