Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina
Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha , Spirotrichonympha , and three new genera Pseudospironympha , Nanospironympha , and Brugerollina
复制标题
Spirotrichonymphea (Parabasalia) 的分子系统发育，重点是 Spironympha、Spirotrichonympha 和三个新属 Pseudospironympha、Nanospironympha 和 Brugerollina
DOI：
10.1111/jeu.12967
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发表时间：
2023
影响因子：
2.2
通讯作者：
Gile, Gillian H.
中科院分区：
文献类型：
--
作者：
Noda, Satoko;Kitade, Osamu;Jasso‐Selles, Daniel E.;Taerum, Stephen J.;Takayanagi, Miki;Radek, Renate;Lo, Nathan;Ohkuma, Moriya;Gile, Gillian H.
螺旋毛蚤属（Spirotrichonymphea）是副基栖动物门（Parabasalia）的6纲之一，以鞭毛多、螺旋状排列为特征，仅存在于白蚁的肠道中。由于复杂的形态进化和分子数据的缺乏，13个描述的属之间的系统发育关系还没有得到很好的理解。一个这样的未充分研究的属是Spiralpha。它一直被认为是一个有效的属，一个亚属Spirotrichonympha，或一个“未成熟”的生活史阶段Spirotrichonympha。为了阐明这一点，我们对从散白蚁和霍白蚁后肠分离的螺旋线虫和螺旋毛线虫的小亚基rRNA基因序列进行了测序，并证实了H. sjostedtisymbions使用荧光原位杂交。螺旋体目前是多系的限制，与bothH。sjostedtisobiont物种分支分别从“真正的“螺从散白蚁。与此相似，H. sjostedtibranches分开的“真正的“Spirotrichonympha发现在散白蚁。我们的数据支持SpironymphafromReticulitermes作为一个有效的属最密切相关的Spirotrichonympha，虽然它的单系和种间关系没有解决在我们的分子系统发育分析。我们提出了三个新的属，以适应H。散白蚁属Spirotrichonympha二新种。
Spirotrichonymphea, one of the six classes of phylum Parabasalia, are characterized by bearing many flagella in spiral rows, and they occur exclusively in the guts of termites. Phylogenetic relationships among the 13 described genera are not well understood due to complex morphological evolution and a paucity of molecular data. One such understudied genus isSpironympha. It has been variously considered a valid genus, a subgenus ofSpirotrichonympha, or an “immature” life cycle stage ofSpirotrichonympha. To clarify this, we sequenced the small subunit rRNA gene sequences ofSpironymphaandSpirotrichonymphacells isolated from the hindguts ofReticulitermesspecies andHodotermopsis sjostedtiand confirmed the molecular identity ofH. sjostedtisymbionts using fluorescence in situ hybridization.Spironymphaas currently circumscribed is polyphyletic, with bothH. sjostedtisymbiont species branching separately from the “true”SpironymphafromReticulitermes. Similarly, theSpirotrichonymphasymbiont ofH. sjostedtibranches separately from the “true”Spirotrichonymphafound inReticulitermes. Our data supportSpironymphafromReticulitermesas a valid genus most closely related toSpirotrichonympha, though its monophyly and interspecific relationships are not resolved in our molecular phylogenetic analysis. We propose three new genera to accommodate theH. sjostedtisymbionts and two new species ofSpirotrichonymphafromReticulitermes.