TS/A - A NEW METASTASIZING CELL-LINE FROM A BALB/C SPONTANEOUS MAMMARY ADENOCARCINOMA
TS/A - A NEW METASTASIZING CELL-LINE FROM A BALB/C SPONTANEOUS MAMMARY ADENOCARCINOMA
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DOI：
10.1007/bf00121199
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发表时间：
1983-01-01
影响因子：
4
通讯作者：
PRODI, G
中科院分区：
文献类型：
--
作者：
NANNI, P;DEGIOVANNI, C;PRODI, G
体外建立了一种转移性小鼠细胞系（TS/A），该细胞系源自 BALB/c 雌性退休种鸡中自发产生的乳腺癌。它表现出显着的形态异质性，这在塑料贴壁培养物（细胞类型从上皮样到成纤维细胞样）以及半固体琼脂培养物中都很明显。 TS/A 系表现出特定细胞质雌二醇受体的存在，其结合活性为 16 fmol/mg 胞质蛋白。体内生长模式如下：a.c.接种 105 个细胞导致同系动物 100% 肿瘤捕获和杀死；平均生存时间为 54 .+-。 1天；在同系动物中没有表现出显着的移植免疫原性；它能够产生自发性肺转移和人工肺集落；它在 H-2 匹配、轻微组织相容性抗原不相容的宿主中具有很高的生长能力（106 个细胞在 58 + 2 天内杀死 100% DBA/2 小鼠）。该自发性乳腺肿瘤细胞系被提议作为研究肿瘤群体与转移扩散、肿瘤免疫原性和乳腺肿瘤治疗相关的异质性的有用模型。
A metastasizing mouse cell line (TS/A), originated from a mammary adenocarcinoma which arose spontaneously in a BALB/c female retired breeder, was established in vitro. It displayed a remarkable morphologic heterogeneity, which is evident in plastic adherent cultures (cell types ranging from epithelial-like to fibroblast-like) as well as in semi-solid agar cultures. The TS/A line exhibited the presence of specific cytoplasmic estradiol receptor, with a binding activity of 16 fmol/mg cytosol protein. The in vivo growth pattern was as follows: a s.c. inoculum of 105 cells caused a 100% tumor take and kill in syngeneic animals; mean survival time was a 54 .+-. 1 days; it did not show significant transplant immunogenicity in syngeneic animals; it was able to give rise to both spontaneous lung metastases and artificial lung colonies; it had a high capacity to grow in H-2 matched, minor histocompatibility antigen incompatible hosts (106 cells killed 100% DBA/2 mice in 58 + 2 days). This line of spontaneous mammary tumor cells is proposed as a useful model for studies on the heterogeneity of the neoplastic population in relation to metastatic spread, on tumor immunogenicity, and on therapy of mammary neoplasia.