Identification and function of 11 Rab GTPases in giant freshwater prawn Macrobrachium rosenbergii
Identification and function of 11 Rab GTPases in giant freshwater prawn Macrobrachium rosenbergii
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罗氏沼虾11种Rab GTP酶的鉴定及功能
DOI：
10.1016/j.fsi.2014.12.021
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发表时间：
2015-03-01
影响因子：
4.7
通讯作者：
Ren, Qian
中科院分区：
文献类型：
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作者：
Huang, Ying;Ren, Qian
Rab GTPases是Ras样GTPases超家族的成员，是内吞膜运输的中心元件。然而，罗氏沼虾中Rab基因的研究却知之甚少。本研究共鉴定出11个Rab基因。罗森伯托。所有的MrRabs都有一个RAB域。系统发育分析表明，这11个MrRabs可分为不同的类群。MrRab基因在心脏、血细胞、肝胰腺、鳃、胃和肠中普遍表达。实时荧光定量PCR结果显示，白色斑点综合征病毒（WSSV）感染对虾后，MrRab基因表达显著上调，表明MrRab基因可能在对虾天然免疫应答中发挥重要作用。此外，在副溶血性弧菌（Vibrio parahaemolyticus）的攻击下，对虾肝胰腺中MrRabs的表达水平也显著上调，这可能表明MrRabs参与了对虾的抗菌免疫。这些初步结果表明MrRabs参与了M.罗森伯托。(C)2014爱思唯尔有限公司版权所有。
Rab GTPases, members of the Ras-like GTPase superfamily, are central elements in endocytic membrane trafficking. However, little is known of the Rab genes in the giant freshwater prawn Macrobrachium rosenbergii. In this study, 11 Rab genes were identified from M. rosenbergii. All MrRabs have a RAB domain. Phylogenetic analysis showed that these 11 MrRabs were divided into different groups. The MrRab genes were ubiquitously expressed in heart, hemocytes, hepatopancreas, gills, stomach, and intestines. Real-time polymerase chain reaction revealed that the MrRab genes were significantly upregulated by white spot syndrome virus (WSSV) in the prawns, indicating that MrRabs might play an important role in innate immune response against WSSV. Moreover, after challenge with Vibrio parahaemolyticus, the expression levels of all MrRabs in the hepatopancreas were also upregulated, which might indicated the involvement of MrRabs in prawns antibacterial immunity. In all, these preliminary results showed that MrRabs were involved in innate immunity of M. rosenbergii. (C) 2014 Elsevier Ltd. All rights reserved.