Selective metabolite and peptide capture/mass detection using fluorous affinity tags
Selective metabolite and peptide capture/mass detection using fluorous affinity tags
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DOI：
10.1021/pr060608s
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Siuzdak, Gary
中科院分区：
文献类型：
--
作者：
Go, Eden P.;Uritboonthai, Wilasinee;Siuzdak, Gary
描述了一种新的和通用的方法，用于具有各种化学功能的高度复杂的生物来源的混合物的样品子集的有针对性的富集和随后的直接质谱表征。具体地，首先用全氟烷基选择性地标记含有感兴趣的化学部分的样品组分，然后将整个样品施加到全氟烷基甲硅烷基化的多孔硅（pSi）表面。由于全氟化标签独特的疏水性和疏脂性，使用简单的表面洗涤可以很容易地去除未标记的样品组分，然后可以通过硅上解吸/电离质谱法（DIOS-MS）直接分析富集的样品部分。重要的是，这种基于氟的富集方法提供了一个单一的平台，同样适用于肽以及小分子集中的应用。该技术的实用性证明了富集和质谱分析的各种肽子集从蛋白质和血清中的氨基酸。
A new and general methodology is described for the targeted enrichment and subsequent direct mass spectrometric characterization of sample subsets bearing various chemical functionalities from highly complex mixtures of biological origin. Specifically, sample components containing a chemical moiety of interest are first selectively labeled with perfluoroalkyl groups, and the entire sample is then applied to a perfluoroalkyl-silylated porous silicon (pSi) surface. Due to the unique hydrophobic and lipophobic nature of the perfluorinated tags, unlabeled sample components are readily removed using simple surface washes, and the enriched sample fraction can then directly be analyzed by desorption/ionization on silicon mass spectrometry (DIOS-MS). Importantly, this fluorous-based enrichment methodology provides a single platform that is equally applicable to both peptide as well as small molecule focused applications. The utility of this technique is demonstrated by the enrichment and mass spectrometric analysis of both various peptide subsets from protein digests as well as amino acids from serum.