Tobamoviruses can be frequently present in the oropharynx and gut of infants during their first year of life
Tobamoviruses can be frequently present in the oropharynx and gut of infants during their first year of life
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DOI：
10.1038/s41598-020-70684-w
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发表时间：
2020-08-12
影响因子：
4.6
通讯作者：
Arias, Carlos F.
中科院分区：
文献类型：
--
作者：
Aguado-Garcia, Yarenci;Taboada, Blanca;Arias, Carlos F.
据报道，植物病毒在成年人的肠道中很常见，可能是食物摄入的结果。在这项工作中，我们报告说，植物病毒也可以经常发现在肠道和口咽部的儿童在他们的生命的第一年，即使他们是完全母乳喂养。从出生到1岁，每月从一个半农村社区的3名明显健康的儿童中收集粪便和口咽样本，并通过下一代测序进行分析。在100%的粪便样本和65%的口咽样本中，至少鉴定出一种植物病毒。Virgaviridae家族中的烟草花叶病毒是迄今为止最常检测到的，热带苏打苹果花叶病毒、胡椒轻度斑驳病毒和仙人掌花叶病毒2是最常见的物种。可以组装17个完整的病毒基因组，系统发育分析显示，在人群中传播的病毒株的多样性很大。这些结果表明，儿童持续暴露于广泛和高度多样化的病毒。早期常见的植物病毒是否会直接或通过与微生物群其他成员的相互作用影响婴儿的免疫系统，仍有待研究。
Plant viruses have been reported to be common in the gut of human adults, presumably as result of food ingestion. In this work, we report that plant viruses can also be found frequently in the gut and oropharynx of children during their first year of life, even when they are exclusively breast-fed. Fecal and oropharynx samples were collected monthly, from birth to 1 year of age, from three apparently healthy children in a semi-rural community and analyzed by next generation sequencing. In 100% of the fecal samples and 65% of the oropharynx samples at least one plant virus was identified. Tobamoviruses in the Virgaviridae family were by far the most frequently detected, with tropical soda apple mosaic virus, pepper mild mottle virus, and opuntia tobamovirus 2 being the most common species. Seventeen complete virus genomes could be assembled, and phylogenetic analyses showed a large diversity of virus strains circulating in the population. These results suggest that children are continuously exposed to an extensive and highly diverse collection of tobamoviruses. Whether the common presence of plant viruses at an early age influences the infant's immune system, either directly or through interaction with other members of the microbiota, remains to be investigated.