Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
复制标题
环状 RNA-0001283 通过 MiR-187/HIPK3 轴抑制乳腺癌增殖和侵袭
DOI：
10.12659/msm.921502
复制
发表时间：
2020-02-18
影响因子：
3.1
通讯作者：
Jiang, Yaling
中科院分区：
文献类型：
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作者：
Hu, Ying;Guo, Feixiang;Jiang, Yaling
背景：环状RNA（CircRNA）是参与乳腺癌发生发展的关键调控因子。材料/方法：采用高通量测序技术检测乳腺癌组织中circRNA-0001283基因的表达水平，采用实时定量PCR和Western-blot技术检测circRNA-0001283蛋白的表达。采用CCK-8试剂盒和流式细胞仪检测细胞活力和细胞凋亡。Transwell侵袭实验检测细胞的侵袭能力。结果：circRNA-0001283在乳腺癌组织中表达下调。circRNA-0001283的异位表达显著抑制乳腺癌细胞的存活和侵袭，并诱导细胞凋亡。此外，circRNA-0001283与miR-187结合并降低miR-187的表达，这导致细胞生长和侵袭的抑制。结论：本研究结果揭示了乳腺癌中一种新的功能性circRNA-0001283，为乳腺癌的治疗提供了新的靶点。
Background: Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer.Material/Methods: Breast cancer tissue samples were tested using high-throughput sequencing to identify the levels of relative genes; and proteins were addressed by using quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot. Cell ability and cell apoptosis were investigated by Cell Counting Kit-8 (CCK-8) and flow cytometry. Invasion was detected by Transwell invasion assay. The identification of target genes was analyzed by dual-luciferase reporter assay.Result: Downregulation of circRNA-0001283 expression was observed in breast cancer tissue samples. Ectopic expression of circRNA-0001283 remarkably suppressed cell viability and invasion, and induced apoptosis in breast cancer cells. Furthermore, circRNA-0001283 bound to miR-187 and decreased the expression of miR-187, which resulted in inhibition in cell growth and invasion. Finally, we showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187.Conclusions: The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer.