Scutellarin ameliorates the stress-induced anxiety-like behaviors in mice by regulating neurotransmitters
Scutellarin ameliorates the stress-induced anxiety-like behaviors in mice by regulating neurotransmitters
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灯盏乙素通过调节神经递质改善小鼠应激诱发的焦虑样行为
DOI：
10.1002/ptr.7106
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发表时间：
2021-04-15
影响因子：
7.2
通讯作者：
Zhao, Ming-gao
中科院分区：
文献类型：
--
作者：
Guo, Yan-yan;Zhou, Ying;Zhao, Ming-gao
焦虑症是患者常见的精神症状，会导致日常生活中断。灯盏乙素 (Scu) 是灯盏花的主要成分，已被用作对抗谷氨酸诱导的兴奋性毒性的神经保护剂。然而，Scu 对应激相关神经心理疾病的潜在影响尚未明确。在这项研究中，小鼠的焦虑样行为是由急性束缚应激引起的。 Scu腹膜内注射（每天两次，共3天）。结果表明，Scu 通过降低递质释放水平对小鼠表现出良好的保护活性。束缚压力会导致小鼠出现明显的焦虑样行为。 Scu的治疗可以显着改善高架十字迷宫中开臂的移动时间和旷场测试的中心时间。 Scu 治疗抑制了应激小鼠的动作电位放电频率，恢复了过度的突触前量子释放，并下调了前额皮质 (PFC) 中谷氨酸能受体的表达水平。 Scu治疗几乎消除了小鼠脑PFC组织中的GABA(A)R α1和GABA(A)gamma(2)表达。在压力诱发的焦虑小鼠中，压力会增加微型兴奋性突触后电流 (mEPSC) 的频率，而 Scu 治疗可以逆转这种情况。因此，Scu 具有有效的抗焦虑活性，对于治疗应激引起的焦虑症可能有价值。
Anxiety disorders are a common frequently psychiatric symptom in patients that lead to disruption of daily life. Scutellarin (Scu) is the main component of Erigeron breviscapus, which has been used as a neuroprotective agent against glutamate-induced excitotoxicity. However, the potential effect of Scu on the stress-related neuropsychological disorders has not been clarified. In this study, Anxiety-like behavior was induced by acute restraint stress in mice. Scu were injected intraperitoneally (twice daily, 3 days). Results showed that Scu exhibited good protective activity on mice by decreasing transmitter release levels. Restraint stress caused significant anxiety like behavior in mice. Treatment of Scu could significantly improve the moving time of open arms in Elevated Plus Maze and central time on open field test. Scu treatment suppressed action potential firing frequency, restored excessive presynaptic quantal release, and down-regulated glutamatergic receptor expression levels in the prefrontal cortex (PFC) of stressed mice. GABA(A)R alpha 1 and GABA(A)gamma(2) expression in the brain PFC tissues of mice were nearly abrogated by Scu treatment. In stress-induced anxiety mice, stress can increase the frequency of mini excitatory postsynaptic currents (mEPSC), which can be reversed by Scu treatment. Therefore, Scu has a potent anxiolytic activity and may be valuable for the treatment of stress-induced anxiety disorders.