SPORE STRUCTURE AS REVEALED BY THIN SECTIONS
SPORE STRUCTURE AS REVEALED BY THIN SECTIONS
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DOI：
10.1128/jb.66.3.300-311.1953
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发表时间：
1953-01-01
影响因子：
3.2
通讯作者：
ROBINOW, CF
中科院分区：
文献类型：
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作者：
ROBINOW, CF
蜡状芽孢杆菌和B的休眠孢子的0.3 - 0.4 [mu]厚切片。Giemsa染色后在光学显微镜下观察。在电子显微镜下研究未染色切片。在B。巨大芽孢是一种薄的、不太光滑的皮层，在电子显微镜下是透明的，直接出现在孢子衣下面。在B中不能确定地检测到。蜡状孢子这两种孢子的核心。由嗜碱性物质组成，在电子显微照片中非常致密和均匀。在用[image]/HCl在60[degree]下水解，或用[image]/1 HClO 4处理或用核糖核酸酶消化（选择的方法）提取大部分嗜碱性物质后，核元件的新月或环可以在核心的表面但在核心内染色。休眠孢子中核物质的排列类似于常规方法先前在萌发早期固定的整个孢子中揭示的核环构型。没有证据已经获得了一个额外的细胞质核的存在，在休眠孢子已假定由作者。B的电子显微照片。用1/1HNO 3处理后的巨大孢子切片显示，核物质通过皮层和内部孢子涂层的间隙向外流动。费尔根水解和类似的方法在整个孢子中总是显示出偏心和突出的核，现在必须被认为是不可避免的人工制品，是休眠阶段的特征。P. C博士的观察Fitz-James用微小的玻璃珠振摇破裂的孢子，得到了非常相似的结果。
Sections, 0.3 _ 0.4 [mu] thick, of resting spores of Bacillus cereus and B. megaterium were examined in the light microscope after Giemsa staining. Unstained sections were studied in an electron microscope. In B. megaterium spores, a thin, poorly stainable cortex, transparent in electron-micrographs, occurs immediately beneath the spore coat. It cannot be detected with certainty in B. cereus spores. The core of the spores of both spp. consists of basiphil matter which is remarkably dense and homogeneous in electron-micrographs. After extraction of most of the basiphil matter by hydrolysis with [image]/HCl at 60[degree], or treatment with [image]/1 HClO4 or digestion with ribonuclease (the method of choice) a crescent or ring of nuclear elements can be stained at the surface of, but within, the core. The arrangement of the nuclear matter in resting spores is similar to the nuclear ring configurations which ordinary methods have revealed previously in whole spores fixed early during germination. No evidence has been obtained for an extra-cytoplasmic nucleus whose existence in resting spores had been postulated by the writer. Electron-micrographs of B. megaterium spores sectioned after treatment with [image]/1 HNO3 reveal an outward flow of the nuclear material through a gap in the cortex and the inner sporecoat. The eccentric and protruding nuclei which Feulgen-hydrolysis and similar procedures invariably reveal in whole spores, must now be regarded as unavoidable artefacts, characteristic of the resting stage. Observations by Dr. P. C. Fitz-James on spores cracked open by shaking them with minute glass beads give very similar results.