Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.
Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.
复制标题
RAPD 遗传标记揭示红三叶草 (Trifolium pratense L.) 育种群体遗传多样性分析。
作者：
Odeth Ulloa;F. Ortega;H. Campos
红三叶草是温带地区重要的饲用豆科植物，对其繁殖群体的遗传结构了解甚少。利用随机扩增多态性DNA （RAPD）遗传标记分析了来自智利、阿根廷、乌拉圭和瑞士的20个育种群体和栽培品种的遗传多样性和变异分布。对所有可能的两两组合计算遗传距离。多态性水平较高，多态位点在群体间的比例为74.2%。一个非认证的群体比其相应的认证的群体显示出更高的多态位点比例。基因多样性值和群体遗传学参数表明所分析的群体具有多样性。分子方差分析（AMOVA）表明，种群内变异比例最大（80.4%）。RAPD标记是红三叶草育种的有效工具。基于遗传距离的树状图将所分析的繁殖种群分为三个不同的群体。所观察到的多样性的数量和分配对鉴定合成品种亲本的来源群体和利用所分析群体中的变异有价值。
Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and cultivars from Chile, Argentina, Uruguay, and Switzerland. Genetic distances were calculated for all possible pairwise combinations. A high level of polymorphism was found and the proportion of polymorphic loci across populations was 74.2%. A population derived from a non-certified seedlot displayed a higher proportion of polymorphic loci than its respective certified seedlot. Gene diversity values and population genetics parameters suggest that the populations analyzed are diverse. An analysis of molecular variance (AMOVA) revealed that the largest proportion of variation (80.4%) resides at the within population level. RAPD markers are a useful tool for red clover breeding programs. A dendrogram based on genetic distances divided the breeding populations analyzed into three distinct groups. The amount and partition of diversity observed can be of value in identifying the populations that parents of synthetic cultivars are derived from and to exploit the variation available in the populations analyzed.