NMR solution structure and conformational analysis of the calcium release agent cyclic adenosine 5'-diphosphate ribose (cADPR).
NMR solution structure and conformational analysis of the calcium release agent cyclic adenosine 5'-diphosphate ribose (cADPR).
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钙释放剂环腺苷 5-二磷酸核糖 (cADPR) 的 NMR 溶液结构和构象分析。
DOI：
10.1081/ncn-100002079
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Pope,SC
中科院分区：
文献类型：
--
作者：
Graham,SM;Pope,SC
用高分辨核磁共振研究了钙释放剂cADPR的溶液结构。假旋转分析表明，cADPR的糖环在溶液中主要（约75%）为South构象，A环和N环分别为2 T3（C2′-endo（major）-C3′-exo（minor））和43 T（C3′-exo-C4′-endo）构象.还测定了主链扭转角β和γ。虽然未观察到在cADPR的晶体结构中的小北构象，主要的南构象的解决方案值比较以及在晶体结构中发现的那些。
A high-resolution NMR study of the solution structure of the calcium release agent cADPR has been performed. Pseudorotationals analysis reveals that in solution both sugar rings in cADPR adopt predominantly (∼75%) South conformations, with the A and N rings adopting approximately2T3(C2′-endo(major)-C3′-exo(minor) and43T (C3′-exo-C4′-endo) conformations, respectively. The backbone torsion angles β and γ have also been determined. While the minor North conformers were not observed in the crystal structure of cADPR, the solution values of the major South conformers compare well to those found in crystal structure.