Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.
Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.
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DOI：
10.1083/jcb.120.3.825
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发表时间：
1993-02
期刊：
影响因子：
--
通讯作者：
Knudson CB
中科院分区：
文献类型：
--
作者：
Knudson CB
细胞外基质的初始组装发生在紧邻软骨细胞表面的区域内，称为细胞相关基质或细胞周基质。在细胞周围基质隔室内的组装需要发生特定的细胞-基质相互作用，其通过膜受体介导。本研究的重点是阐明组装和保留的软骨细胞周围基质蛋白多糖聚集体的重要基质组织的机制。新合成的软骨细胞周基质的组装受到抑制，除了透明质酸六糖，透明质酸的结合，其细胞表面受体的竞争性抑制剂。完全组装的软骨细胞周基质也使用透明质酸六糖置换。当外源性透明质酸加入到无基质的软骨细胞中与聚集蛋白聚糖组合时，在孵育的30分钟内形成了大小与内源性基质相当的细胞周基质。将透明质酸和聚集蛋白聚糖添加到戊二醛固定的软骨细胞中导致与活软骨细胞相当的基质组装。这些基质可以通过加入过量的透明质酸六糖来抑制组装，或者一旦组装后通过随后与透明质酸六糖孵育来置换。结果表明，富含聚集蛋白聚糖的软骨细胞细胞周基质不仅在透明质酸的支架上，而且实际上通过透明质酸和透明质酸受体之间的相互作用锚定到细胞表面。
Initial assembly of extracellular matrix occurs within a zone immediately adjacent to the chondrocyte cell surface termed the cell- associated or pericellular matrix. Assembly within the pericellular matrix compartment requires specific cell-matrix interactions to occur, that are mediated via membrane receptors. The focus of this study is to elucidate the mechanisms of assembly and retention of the cartilage pericellular matrix proteoglycan aggregates important for matrix organization. Assembly of newly synthesized chondrocyte pericellular matrices was inhibited by the addition to hyaluronan hexasaccharides, competitive inhibitors of the binding of hyaluronan to its cell surface receptor. Fully assembled chondrocyte pericellular matrices were displaced using hyaluronan hexasaccharides as well. When exogenous hyaluronan was added to matrix-free chondrocytes in combination with aggrecan, a pericellular matrix equivalent in size to an endogenous matrix formed within 30 min of incubation. Addition of hyaluronan and aggrecan to glutaraldehyde-fixed chondrocytes resulted in matrix assembly comparable to live chondrocytes. These matrices could be inhibited from assembling by the addition of excess hyaluronan hexasaccharides or displaced once assembled by subsequent incubation with hyaluronan hexasaccharides. The results indicate that the aggrecanrich chondrocyte pericellular matrix is not only on a scaffolding of hyaluronan, but actually anchored to the cell surface via the interaction between hyaluronan and hyaluronan receptors.