AN IMPROVED EXTRACTION METHOD TO INCREASE DNA YIELD FROM MOLTED FEATHERS
AN IMPROVED EXTRACTION METHOD TO INCREASE DNA YIELD FROM MOLTED FEATHERS
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DOI：
10.1525/cond.2008.8586
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发表时间：
2008-11-01
期刊：
影响因子：
3.1
通讯作者：
Antolin, Michael F.
中科院分区：
文献类型：
--
作者：
Bayard De Volo, Shelley;Reynolds, Richard T.;Antolin, Michael F.
为了评估蜕皮羽毛作为北方苍鹰（鹰）遗传研究的非侵入性DNA来源的价值，我们从蜕皮羽毛中分离和定量DNA，并比较了五种羽毛类型的产量。我们还比较了PCR的成功率在相同的五个羽毛类型使用五个不同大小的微卫星遗传标记。此外，我们比较了一种常用的提取方法与我们修改以增加DNA产量的方法的DNA产量。结果表明，蜕皮羽毛提供了平均24 ng mul（-1）的DNA，这是一个相对较高的DNA产量相比，其他非侵入性组织来源。尾羽产生的DNA明显多于初级、次级和较小的羽毛，但所有羽毛大小的PCR成功率都一样高。虽然我们改进的提取方法增加了处理羽毛所需的时间，但与未修改的方案相比，它导致了显着更高的DNA产量。
To assess the value of molted feathers as a noninvasive source of DNA for genetic studies of Northern Goshawks (Accipiter gentilis), we isolated and quantified DNA from molted feathers and compared yields across five feather types. We also compared PCR success across the same five feather types using five microsatellite genetic markers of varying size. In addition, we compared DNA yields from a commonly used extraction method versus one we modified to increase DNA yield. Results indicated molted feathers provided on average 24 ng mu l(-1) of DNA, which is a relatively high DNA yield compared to other noninvasive tissue sources. Tail feathers yielded significantly more DNA than primary, secondary, and smaller feathers, yet all feather sizes produced equally high rates of PCR success. Although our modified extraction method increased the time required for processing feathers, it resulted in significantly higher yields of DNA as compared to the unmodified protocol.