Placental apoptotic markers are associated with placental morphometry
Placental apoptotic markers are associated with placental morphometry
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DOI：
10.1016/j.placenta.2021.08.051
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发表时间：
2021-11-01
期刊：
影响因子：
3.8
通讯作者：
Joshi, Sadhana
中科院分区：
文献类型：
--
作者：
Kasture, Vaishali;Sundrani, Deepali;Joshi, Sadhana
简介：先兆子痫是一种影响母亲和胎儿的高血压疾病，是孕产妇和新生儿发病率和死亡率的主要原因。异常胎盘形成是先兆子痫的常见特征，导致胎盘功能障碍。这可能是增加同型半胱氨酸和氧化应激影响细胞凋亡先兆子痫。胎盘细胞凋亡增加可能通过破坏胎盘结构而加重先兆子痫的症状。目前的研究旨在探讨各种胎盘凋亡标记物与先兆子痫妇女胎盘尺寸和母亲及新生儿特征之间的关系。方法：共80例孕妇[先兆子痫组（n = 40）;正常血压对照组（n = 40）]纳入研究。记录胎盘长径、短径、宽度、厚度（中心、脐带附着点和外周）和胎盘重量，ELISA法检测胎盘caspase-3、caspase-8、BAX和Bcl-2蛋白水平，真实的时间定量PCR检测基因表达。测试结果：与对照组相比，先兆子痫组中促凋亡标志物如半胱天冬酶-8和3的蛋白水平较高（p < 0.01），而先兆子痫组中抗凋亡标志物Bcl-2的水平较低（p < 0.05）。Caspase-3和Bcl-2蛋白表达水平与胎盘厚度呈负相关（p < 0.01）。caspase-8和caspase-3蛋白水平与胎盘MDA水平呈正相关（p < 0.01）。Caspase-8与婴儿长度呈负相关（p = 0.055）。讨论：本研究表明各种凋亡标志物与氧化应激和胎盘尺寸的相关性。
Introduction: Preeclampsia is a hypertensive disorder affecting both mother and the fetus and is a major cause of maternal and neonatal morbidity and mortality. Abnormal placentation is a common feature in preeclampsia that contributes to placental dysfunction. It is likely that increased homocysteine and oxidative stress influence apoptosis in preeclampsia. Increased placental apoptosis may aggravate the symptoms of preeclampsia through disruption of the placental structure. The current study aims to examine the association between various placental apoptotic markers with placental dimensions and maternal and neonatal characteristics in women with preeclampsia. Methods: A total of 80 pregnant women [preeclampsia (n = 40); normotensive control (n = 40)] were included in the study. Placental characteristics such as its major axis, minor axis, breadth, thickness (at centre, cord insertion and periphery) and trimmed placental weight were recorded.Placental protein levels of caspase-3, caspase-8, BAX and Bcl-2 were estimated by ELISA and gene expression were examined by real time quantitative PCR. Result: Protein levels of proapoptotic markers such as caspase-8 and 3 were higher (p < 0.01) in the preeclampsia group compared to control whereas, the level of antiapoptotic marker Bcl-2 (p < 0.05) was lower in the preeclampsia group. Caspase-3 and Bcl-2 protein levels were negatively associated with thickness of placenta at cord insertion (p < 0.01). Protein levels of caspase-8 and caspase-3 were positively associated with placental MDA levels (p < 0.01). Caspase-8 was negatively associated with baby length (p = 0.055). Discussion: This study demonstrates the association of various apoptotic markers with oxidative stress and placental dimensions.