Overexpression of Nuclear Protein Kinase CK2 β Subunit and Prognosis in Human Gastric Carcinoma
Overexpression of Nuclear Protein Kinase CK2 β Subunit and Prognosis in Human Gastric Carcinoma
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DOI：
10.1245/s10434-010-0911-9
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发表时间：
2010-06-01
影响因子：
3.7
通讯作者：
Uen, Yih-Huei
中科院分区：
文献类型：
--
作者：
Lin, Kai-Yuan;Fang, Chia-Lang;Uen, Yih-Huei
胃癌是世界上最常见的恶性肿瘤之一，但对其发生发展的分子机制知之甚少。为探讨细胞核蛋白激酶CK 2 β亚基（CK 2 β）表达与胃癌临床病理参数及患者生存期的关系，采用免疫组化法检测了104例胃癌组织中CK 2 β的表达水平。采用配对t检验分析同一患者肿瘤组织与非肿瘤组织细胞核CK 2 β表达的差异。采用双尾卡方检验确定细胞核CK 2 β表达与临床病理参数之间差异的显著性。采用Kaplan-Meier方法绘制了根据各种临床病理参数的所有至事件发生时间终点，然后采用单变量对数秩检验确定显著性。采用考克斯比例风险模型分析细胞核CK 2 β表达对胃癌预后的独立影响，细胞核CK 2 β过表达与胃癌浸润深度显著相关（P = 0.042）。细胞核CK 2 β高表达患者的总生存率显著低于细胞核CK 2 β低表达患者（P = 0.0006）。多因素考克斯回归分析显示，细胞核CK 2 β过表达和分期是胃癌的独立预后指标（P值分别为0.0036和0.0005），细胞核CK 2 β过表达可作为预测胃癌预后的一个有用指标。
Gastric carcinoma is one of the most common malignancies in the world, yet little is known about the molecular process of its development and progression. The aims of this study are to correlate the expression of nuclear protein kinase CK2 beta subunit (CK2 beta) with clinicopathologic parameters and patient survival.Expression levels of nuclear CK2 beta were analyzed in 104 gastric tissues from patients with gastric carcinoma by immunohistochemistry. A paired t test was used to analyze the differences in nuclear CK2 beta expression between tumor and nontumor tissues in the same patient. A two-tailed chi (2) test was performed to determine the significance of the difference between nuclear CK2 beta expression and clinicopathologic parameters. All time-to-event endpoints according to various clinicopathologic parameters were plotted by Kaplan-Meier method, and significance was then determined by univariate log-rank test. Cox proportional-hazards model was used for multivariate analysis to determine the independence of prognostic impact of nuclear CK2 beta expression.Overexpression of nuclear CK2 beta was significantly correlated with depth of invasion (P = 0.042). Patients with high expression levels of nuclear CK2 beta had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2 beta (P = 0.0006). On multivariate Cox regression analysis, overexpression of nuclear CK2 beta and stage were proven to be independent prognostic markers for gastric carcinoma (P = 0.0036 and 0.0005, respectively).Overexpression of nuclear CK2 beta can be a useful marker for predicting the outcome of patients with gastric carcinoma.