Immunoglobulin G binding protein (IGBP) from Rhipicephalus haemaphysaloides: identification, expression, and binding specificity
Immunoglobulin G binding protein (IGBP) from Rhipicephalus haemaphysaloides: identification, expression, and binding specificity
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DOI：
10.1007/s00436-014-4115-2
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发表时间：
2014-12-01
影响因子：
2
通讯作者：
Zhou, Jinlin
中科院分区：
文献类型：
--
作者：
Gong, Haiyan;Qin, Shunqing;Zhou, Jinlin
作为仅次于蚊子的第二重要的人类体外寄生虫，蜱威胁着畜牧业的发展，甚至威胁着全世界人类的健康。免疫球蛋白G结合蛋白（IGBP）被认为是蜱用来逃避宿主免疫系统和宿主抗体造成的损害的主要因素。在这项研究中，在蜱扇头蜱中鉴定出了 IGBP-MB 同源物，该同源物主要在雄性蜱的唾液腺和血淋巴中检测到。重组IGBP (rIGBP/His) 对兔和猪的IgG 表现出显着的结合活性，并与兔IgG 的F(ab)'2 片段结合，但不与Fc 片段结合。尽管蜱中 IGBP 表达的沉默对其吸血和随后的产卵没有明显影响，但 rIGBP/GST 产生的抗体降低了饱足体重（对照组为 218.9 +/- 20 mg，而测试组为 142.5 +/- 43.3 mg，P
As the second most important human ectoparasite, ranked only after mosquitoes, the tick threatens the development of husbandry and even the health of humans worldwide. Immunoglobulin G binding proteins (IGBPs) are considered to be the major factors used by ticks to evade the host immune system and the damage caused by host antibodies. In this study, an IGBP-MB homologue was identified in the tick Rhipicephalus haemaphysaloides, which was predominantly detected in the salivary glands and hemolymph of male ticks. Recombinant IGBP (rIGBP/His) displayed significant binding activity to IgGs from rabbits and pigs, and bound to the F(ab)'2 but not the Fc fragment of rabbit IgG. Although the silencing of IGBP expression in ticks had no obvious effect on their blood-feeding and subsequent oviposition, antibodies raised to rIGBP/GST reduced the replete body weight (218.9 +/- 20 mg in the control group vs. 142.5 +/- 43.3 mg in the test group, P